壳聚糖微球负载的己二胺型聚酰胺-胺树状大分子的制备及对胆红素的吸附  

Preparation of Hexanediamine-contained PAMAM Dendrimers Supported on Chitosan Microspheres and Their Application in the Adsorption of Bilirubin

作　　者：吴丽平[1,2] 张政朴[1] 郭义[2] 王勇[3] 陆伟[3] 

机构地区：[1]南开大学高分子研究所,功能高分子材料教育部重点实验室,天津300071 [2]天津中医药大学针灸学院,医用化学传感器国家重点实验室,天津300193 [3]天津市海河医院,天津300150

出　　处：《高等学校化学学报》2011年第6期1436-1444,共9页Chemical Journal of Chinese Universities

基　　金：天津市应用基础及前沿技术研究计划(批准号:08JCYBJC11300)资助

摘　　要：通过反相悬浮反应制备了戊二醛交联的壳聚糖(CS)微球,以CS微球为载体,合成了己二胺型低代数聚酰胺-胺(Polyamidoamine,PAMAM)树枝状大分子(Genaration≤3).考察了该微球在生理条件下对水溶液中胆红素的吸附行为,探讨溶液的pH值、离子强度、温度、胆红素初始浓度和牛血清白蛋白等因素对吸附的影响.结果表明,吸附剂对胆红素具有良好的吸附性能,CS-G2.0,CS-G3.0,CS-G1.0,CS-G0和CS微球的平衡吸附率分别为94.61%,93.44%,92.97%,86.47%和52.38%;CS-G1.0～CS-G3.0微球在0.5 h的吸附率已经超过70%,1 h基本接近平衡,对胆红素的吸附量高达42.78 mg/g.Glutaraldehyde cross-linked chitosan microspheres（CS） were prepared by inverse phase suspension reaction.Low generations（Gn,n≤3） hexanediamine-contained PAMAM dendrimers were supported to chitosan microspheres.Bilirubin adsorption on the above microspheres was carried out in 0.05 mol/L phosphate buffer solution（pH=7.2-7.4） at 37 ℃.The results show that the equilibrium adsorption percentage of CS-G2.0,CS-G3.0,CS-G1.0,CS-G0 and CS microspheres are 94.61%,93.44%,92.97%,86.47% and 52.38%,respectively.The adsorption percentage of CS-G1.0-CS-G3.0 microspheres is over 70% at 0.5 h and arrived to the equilibrium at about 1 h.The adsorption capacity is up to 42.78 mg/g.All the moderations（CS-G0-CS-G3.0） are much better than CS microspheres for bilirubin adsorption.The effects of temperature,pH,bilirubin initial concentration,ion strength and bovine serum albumin（BSA） on the adsorption properties for bilirubin were studied.It was shown that adsorption percentage increased at higher temperature.Adsorption properties were the best at near neutral solutions of pH≈7.PAMAM dendrimers supported in chitosan microspheres had satisfactory competitive abilities with BSA for bilirubin adsorption though adsorption percentage decreaed to a certain extent in the presence of BSA.

关 键 词：胆红素吸附 壳聚糖微球 己二胺 丙烯酸甲酯 聚酰胺-胺 

分 类 号：O631[理学—高分子化学]