内异方对子宫内膜异位症IL-6、IL-10的调节作用  

The regulating role of Nei Yi Fang on interleukin 6 and interleukin 10 in endometriosis

作　　者：俞超芹[1] 刘玉环[2] 彭新萍[3] 李瑾[1] 潘瑞萍[1] 凌昌全[1] 

机构地区：[1]第二军医大学长海医院中医科,上海200433 [2]第二军医大学长海医院妇产科 [3]湖北省宜昌市第一人民医院妇产科

出　　处：《第二军医大学学报》1999年第9期621-623,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金;国家中医药管理局青年基金

摘　　要：目的：探讨中药内异方对子宫内膜异位症细胞因子 Ｉ Ｌ６、 Ｉ Ｌ１０ 的调节作用。方法：根据 Ｃｕｍ ｍ ｉｎｇｓ Ａ Ｍ 方法建立子宫内膜异位症动物模型，采用 Ｅ Ｌ Ｉ Ｓ Ａ 法检测 Ｉ Ｌ６、 Ｉ Ｌ１０ 含量。结果：非治疗组的腹腔巨噬细胞数量及巨噬细胞在脂多糖（ Ｌ Ｐ Ｓ）刺激下产生的 Ｉ Ｌ６ 含量显著高于正常对照组；内异方治疗组异位种植内膜组织质量、腹腔巨噬细胞数量及巨噬细胞在 Ｌ Ｐ Ｓ刺激下产生的 Ｉ Ｌ６ 水平显著低于非治疗组；非治疗组腹腔巨噬细胞在 Ｌ Ｐ Ｓ刺激下产生的 Ｉ Ｌ１０ 含量显著低于正常对照组和内异方组。结论：子宫内膜异位症细胞因子 Ｉ Ｌ６、 Ｉ Ｌ１０ 的失衡与疾病的发生发展有关。内异方通过抑制 Ｉ Ｌ６ 的合成、促进 Ｉ Ｌ１０ 的分泌，从而阻止了异位内膜组织的生长。Objective: To investigate the regulating role of Nei Yi Fang (NYF) on cytokines in endometriosis. Methods: According to Cummings AM method, animal models with endometriosis was established. IL 6 and IL 10 levels were determined by double antibody sandwich ELISA. Results: The peritoneal macrophage amounts and the production of IL 6 by LPS activated peritoneal macrophages were remarkably higher in untreated group than that in the control group. The weights of endometriotic tissue and peritoneal macrophage amounts were remarkably lower in treated group compared with untreated group, and the levels of IL 6 by LPS activated peritoneal macrophages were significantly reduced. The LPS stimulated IL 10 secretion in peritoneal macrophages from untreated group was significantly lower compared with control group and treated group. Conclusion: The lost balance of IL 6 and IL 10 levels in endometriosis may be related to the pathogenesis of the disease. The treated mechanism of NYF was mediated by restraint of IL 6 composition, and promotion of IL 10 secretion.

关 键 词：子宫内膜异位症 内异方 中医药疗法 IL-6 IL-10 

分 类 号：R271.917.1[医药卫生—中西医结合] R285.5[医药卫生—中医妇科学]