高体积分数氧致慢性肺疾病新生大鼠肺组织血小板源性生长因子的动态表达  

Dynamic Expression of Platelet-Derived Growth Factor in Lung Tissue of Newborn Rats with Hyperoxia-Induced Chronic Lung Disease

作　　者：岳冬梅[1] 薛辛东[1] 

机构地区：[1]中国医科大学附属盛京医院儿科,沈阳110004

出　　处：《实用儿科临床杂志》2011年第10期789-791,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(30672253)

摘　　要：目的探讨高体积分数氧(高氧)损伤状态下,新生大鼠肺组织内血小板源性生长因子(P;;DOI:10.3969/j.issn.GF)的动态变化。方法新生Wistar大鼠320只。依据吸氧体积分数(FiO2)分为实验1组(FiO2=800 mL.L-1)、实验2组(FiO2=600 mL.L-1)、实验3组(FiO2=400 mL.L-1)及空气对照组(FiO2=210 mL.L-1)。实验第1、3、5、7、14天,无菌取其肺组织,采用免疫组织化学法检测各组肺组织P;;DOI:10.3969/j.issn.GF-A、P;;DOI:10.3969/j.issn.GF-B、P;;DOI:10.3969/j.issn.GF受体(P;;DOI:10.3969/j.issn.GFR)-α、P;;DOI:10.3969/j.issn.GFR-β的表达,MetaMorph软件测定平均光密度值,以表示肺组织P;;DOI:10.3969/j.issn.GF-A、P;;DOI:10.3969/j.issn.GF-B、P;;DOI:10.3969/j.issn.GFR-α、P;;DOI:10.3969/j.issn.GFR-β蛋白的表达水平。应用SPSS11.5软件进行统计学分析。结果实验1组及实验2组第1天肺组织P;;DOI:10.3969/j.issn.GF-B表达均低于空气对照组(Pa<0.05);实验1组第5、7天,实验2组及实验3组第7天肺组织P;;DOI:10.3969/j.issn.GF-B表达均高于空气对照组(Pa<0.05)。P;;DOI:10.3969/j.issn.GFR-β高氧刺激后持续增高,3个实验组第1天、第3天时与空气对照组比较无显著差异,第5、7、14天均明显高于空气对照组(Pa<0.05)。3个实验组P;;DOI:10.3969/j.issn.GF-A、P;;DOI:10.3969/j.issn.GFR-α与空气对照组比较差异无统计学意义。结论吸入高氧使新生大鼠肺组织P;;DOI:10.3969/j.issn.GF-B、P;;DOI:10.3969/j.issn.GFR-β表达增加,是导致早期高氧肺损伤可能途径之一。Objective To explore the dynamic changes of platelet-derived growth factor（PDGF） in lung tissue of newborn rats with hyperoxia-induced lung injury condition.Methods Three hundred and twenty newborn Wistar rats were divided into 4 groups according to different oxygen concentrations（FiO2）： experimental 1 group（FiO2=800 mL·L-1）,experimental 2 group（FiO2=600 mL·L-1）,experimental 3 group（FiO2=400 mL·L-1） and room-air control group（FiO2=210 mL·L-1）.Rats in each group were killed on the 1st,3rd,5th,7th and 14th day respectively during the experiment.The expressions of PDGF-A,PDGF-B,platelet-derived growth factor receptor（PDGFR）-α and PDGFR-β in the lung tissue were observed by means of immunohistochemistry.SPSS 11.5 software was used to analyze the data.Results The expressions of PDGF-B in the lung tissue in experimental 1 group and experimental 2 group were lower than those in the room-air control group on the 1st day（Pa0.05）.The expressions of PDGF-B in the lung tissue in experimental 1 group on the 5th,7th day,and those in experimental 2 group and experimental 3 group on the 7th day,were higher than those in room-air control group（Pa0.05）.The expressions of PDGFR-β in lung tissue in 3 experimental groups continued to increase after oxygen exposure.And expressions of PDGFR-β in the lung tissue in 3 experimental groups had no difference with those in control group on the 1st,3rd day,but they were significantly higher than those in room-air control group on the 5th,7th and 14th day（Pa0.05）.The expressions of PDGF-A and PDGFR-α in 3 experimental groups had no difference compared with those in room-air control group.Conclusion The increment of PDGF-B and PDGFR-β expressions in the lung tissues of newborn rats after oxygen exposure can be one of the causes of hyperoxia-induced lung injury.

关 键 词：高体积分数氧 上皮细胞 血小板源性生长因子 慢性肺损伤 大鼠 新生 

分 类 号：R563[医药卫生—呼吸系统]