转基因抗虫棉中cry1Aa基因表达盒的序列测定和检测  

The Sequence and Detection of the cry1Aa Gene Expression Cassette in Transgenic Bt Cotton

作　　者：孙爻[1] 谢家建[1] 范荫荫[1] 李蕊[1] 彭于发[1] 

机构地区：[1]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室/农业部转基因植物环境安全监督检验测试中心(北京),北京100193

出　　处：《棉花学报》2011年第3期224-227,共4页Cotton Science

基　　金：国家重大基础研究发展规划项目(2007CB109201);转基因新品种培育重大科技项目(2011ZX08012-002)

摘　　要：从转基因抗虫棉南农6号F1中克隆了一种新的Bt基因表达盒—cry1Aa基因表达盒,与已经测定的我国转基因棉花中的cry1Ac基因和cry1Ab/Ac基因的表达盒序列比较发现,它们在Bt基因与7S UTR的连接区域存在较大差异。根据这个差异区域的序列,设计特异性引物对cry1Aa-F/cry1Aa-R,建立了cry1Aa基因表达盒特异性检测方法。在18份来自江苏省的棉花材料中,有8份材料检测出含有cry1Aa基因表达框。建立的构建特异性检测方法为转基因棉花的Bt基因表达盒的鉴别和转基因谱系分析等提供了有效的技术手段。A new Bt gene expression cassette cry1Aa gene expression cassette was identified from transgenic insect-resistance cotton ＂Nannong 6 F1＂ in this study.The difference was found located at the connection region between Bt gene and 7S UTR terminator while being compared with the cry1Ac gene and cry1Ab/Ac gene expression cassettes reported previous.Primer pair cry1Aa-F/cry1Aa-R was designed and construct-specific detection method of cry1Ab/Aa gene expression cassette was established based on the difference.Eight of eighteen cotton varieties from Jiangsu province were identified to have cry1Aa gene expression cassette using this methods.The method established was useful for identification Bt gene expression cassette and analysis of transgenic lineages.

关 键 词：转基因棉花 cry1Aa基因 表达盒 PCR检测 

分 类 号：S562[农业科学—作物学]