新疆一枝蒿与黄花蒿粗提物抗棉铃虫与棉蚜的特性研究  

The Study on Resistance of Artemisia rupestrisand Artemisia annua to Cotton Bollworm (Helicoverpa armigera)and Cotton Aphid (Aphis gossypii)

作　　者：何海[1] 黄丽[1] 张学涛[1] 张雷[1] 李芬[1] 刘小宁[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《新疆农业科学》2011年第5期889-895,共7页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区科技支疆项目(200991119);新疆生物资源基因工程重点实验室项目(XJDX0201-2007-04)

摘　　要：【目的】为利用新疆一枝蒿研制新型无公害植物杀虫药剂奠定基础。【方法】采用97%乙醇对新疆一枝蒿(Artemisia rupestris)和黄花蒿(Artemisia annua)进行了总提,将所得粗提物对棉花重要害虫—棉铃虫(Helicoverpa armigera)与棉蚜(Aphis gossypii)进行了生物活性测定,同时还使用新疆一枝蒿粗提物对棉铃虫开展了室内、室外的驱避实验。【结果】(1)新疆一枝蒿与黄花蒿粗提物对棉铃虫半致死浓度LC50分别为3.515 7与4.781 1 mL/100g;(2)新疆一枝蒿与黄花蒿粗提物对棉蚜半致死浓度LC50分别为0.956与2.047 4 g/100 mL;(3)新疆一枝蒿粗提物对棉铃虫室内室外趋避率分别为28.39%与80.68%。【结论】新疆一枝蒿粗提物对棉铃虫与棉蚜的毒杀作用较好并高于黄花蒿粗提物,且新疆一枝蒿粗提物对棉铃虫有驱避和生长抑制等作用。[ Objective] This research can be a foundation for the development of new pollution-free botanical insecticides. [ Method] Two types of coarse solutions from Artemisia rupestris and Artemisia annua were extracted with 97 % ethanol, and toxicities of the two extracts to cotton bollworm and cotton aphid were determined, and expellent effect of Artemisia rupestris to laying eggs of cotton bollworms in both the laboratory and the field were also studied. [Result] The results showed：（ 1 ） The LC50 of extracts from Artemisia rupestris and Artemisia annua to cotton bollworm are 0.956 and 2.047 4 g/100mL; （2） The LC50 of extracts from Artemisia rupestris and Artemisia annua to cotton aphid are 0.956 and 2.047 4 g/100mL; （3） The repellency of extracts of Artemisia rupestris on laying eggs of cotton bollworm in the laboratory and in the field are 28.39% and 80.68%. [ Conclusion] The findings demonstrate that the extracting coarse solutions from Artemisia rupestris are better than that from Artemisia annua as to the killing effect on cotton bollworm and cotton aphid, and it had an expellent effect on laying eggs of cotton bollworms in both the laboratory and the field.

关 键 词：新疆一枝蒿 黄花蒿 棉铃虫 棉蚜 粗提物 杀虫生物活性 

分 类 号：S481.9[农业科学—农药学]