酵母锌与姜黄素联合对酒精性肝损伤保护作用  

Synergic effect of curcumin and zinc-enriched yeast on chronic alcoholic liver injury in mice

作　　者：张晓畅[1] 包巍[1] 荣爽[1] 郝丽萍[1] 姚平[1] 刘烈刚[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院,湖北武汉430030

出　　处：《中国公共卫生》2011年第6期746-747,共2页Chinese Journal of Public Health

基　　金：教育部新世纪优秀人才支持计划(NCET2004-0707);华中科技大学研究生科技创新基金(HF-06-022-08-513)

摘　　要：目的研究酵母锌与姜黄素的联合作用对酒精性肝损伤的干预效果,为酒精性肝病的预防和治疗提供科学依据。方法将实验小鼠随机分为正常对照、酒精对照、姜黄素+低、高剂量酵母锌4组,梯度酒精暴露6周造酒精肝损伤模型,同时分别给予低、高剂量(2.5、10 mg/kg)酵母锌联合姜黄素(75 mg/kg)灌胃干预。测定血清丙氨酸转氨酶(ALT)、天冬氨酸转移酶(AST)水平,制作肝组织病理切片并测定肝组织总抗氧化能力(T-AOC)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽硫转移酶(GST)、谷胱甘肽过氧化物酶(GSH-PX)含量。结果姜黄素+低、高剂量酵母锌组ALT活力、MDA含量分别为(34.11±26.04)、(41.74±32.75)U/L和(0.99±0.19)、(0.97±0.25)nmol/mg prot,明显低于酒精对照组(81.54±37.56)U/L和(1.32±0.22)nmol/mg prot(P<0.05);姜黄素+高剂量酵母锌组GSH、GST、GSH-PX值分别为(1.03±0.15)、(13.84±2.72)、(37.56±4.67)mg/g prot,高于酒精对照组(P<0.05)。结论酵母锌与姜黄素联合对慢性酒精肝损伤具有良好的保护作用,其防护效应可能与其减轻氧化损伤有关。Objective To study the synergic effect of curcumin and zinc-enriched yeast on chronic alcohol-induced liver injury,and to provide scientific evidence for the prevention and treatment of alcoholic liver disease.Methods Balb/c mice were randomly divided into 4 groups：normal control,ethanol control,curcumin＋low dose zinc-enriched yeast,and curcumin ＋ high dose zinc-enriched yeast group.The chronic alcoholic liver damage model was established with gradient dose of ethanol exposure for 6 weeks.Mice in curcumin ＋ low dose zinc-enriched yeast and curcumin＋high dose zinc-enriched yeast group were administered to 75 mg/kg curcumin supplemented with low dose zinc-enriched yeast（2.5 mg/kg）or high dose zinc-enriched yeast（10 mg/kg）per day.After 6 weeks,the enzyme activity of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）in serum and the level of total antioxidant capacity（T-AOC）,malondialdehyde（MDA）,glutathion（GSH）,glutathione transferase（GST）,and glutathione peroxidase（GSH-PX）in liver tissues were determined and histopathological changes of the liver were observed.Results The level of ALT and MDA in curcumin＋low dose zinc-enriched group and curcumin＋high dose zinc-enriched yeast group were 34.11±26.04,41.74±32.75 U/L,and 0.99±0.19,0.97±0.25 nmol/mg · prot,respectively,and significantly lower than those in the ethanol control group（P0.05）.The level of GSH,GST and GSH-PX in curcumin ＋ high dose zinc-enriched yeast group were 1.03±0.15,13.84±2.72,and 37.56±4.67 mg/g · prot,evidently higher than those in the ethanol control group（P0.05）.Conclusion Curcumin and zinc-enriched yeast pretreatment have protective effects on chronic alcoholic liver injury in mice.The mechanism might be related to the alleviation of oxidative damage.

关 键 词：酵母锌 姜黄素 酒精 肝损伤 氧化应激 

分 类 号：R114[医药卫生—卫生毒理学]