六味地黄丸对OLETF大鼠脂肪组织脂联素mRNA转录水平的影响  

Effects of Liuwei Dihuang Pills on the level of adiponectin mRNA of adipose tissue in OLETF rats

作　　者：郭煜[1] 朱波[1] 李晨钟[1] 潘永华[1] 张燕[1] 薛耀明[1] 

机构地区：[1]南方医科大学南方医院内分泌代谢科,广州市510515

出　　处：《实用医学杂志》2011年第11期1922-1925,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30371839);广东省重点科技攻关项目(编号:2003C34424);广东省科技计划项目(编号:2004B30701011);广东省中医药局科研课题(编号:2009454)

摘　　要：目的:研究六味地黄丸对自发性2型糖尿病大鼠脂肪组织脂联素的影响。方法:OLETF鼠40只,分为干预组和对照组,LETO鼠10只作为正常对照组。干预组于8周龄起以六味地黄丸灌胃给药。监测不同周龄体质量、血糖、血浆胰岛素水平,并于8、32和40周分批宰杀大鼠,检测其脂肪组织脂联素mRNA。结果:8周龄OLETF鼠和LETO鼠脂肪组织脂联素mRNA水平差异无显著性,32和40周时OLETF鼠脂肪组织脂联素mRNA水平显著低于LETO鼠(均P<0.01)。六味地黄丸可显著升高32和40周龄的OELTF鼠脂肪组织脂联素水平(均P<0.05)。OLETF鼠胰岛素敏感指数在32和40周龄显著低于LETO鼠(均P<0.01),六味地黄丸可显著增高胰岛素敏感指数(ISI)(均P<0.05)。各周龄OLETF鼠脂肪组织脂联素mRNA水平与ISI显著相关(P<0.01)。结论:OLETF鼠脂肪组织脂联素mRNA水平与胰岛素敏感性呈正相关。六味地黄丸早期干预能显著增加OLETF鼠脂脂肪组织脂联素mRNA水平,改善OLETF鼠胰岛素抵抗。Objective To investigate the effects of Liuwei Dihuang Pills （LWDHP） on adiponectin （APN） mRNA level of visceral fat in OLETF rats. Methods 40 male Otsuka Long-Evans Tokushima Fatty （OLETF） rats were randomly divided into tow groups （n = 20 per group）. Rats of one group were given LWDHP （2.4 mg/kg.d） by intragastric administration from 8 weeks of age, while the other group given water. 10 male Long-evans Tokushima Otsuka （LETO） rats were made as normal control group. The levels of blood glucose, plasma insulin and adiponectin mRNA in adipose tissue in the rates of different weeks of age were determined. Results The levels of APN mRNA in OLETF rats of 32 and 40 weeks of age were significantly decreased （both P 〈 0.01 ）, the APN mRNA level of the rates of LWDHP group were significantly increased （both P 〈 0.05）. There was a positive correlation between the insulin sensitivity index （ISI）and the level of APN mRNA in the OLETF rates of different weeks of age （P 〈 0.05）. Conclusion The APN mRNA level is positively correlated to insulin sensitivity. LWDHP can increase APN mRNA level of adipose tissue and improve insulin resistance in OLETF rats.

关 键 词：糖尿病 2型 胰岛素抵抗 脂联素 六味地黄丸 

分 类 号：R285.5[医药卫生—中药学]