掌叶覆盆子化学成分的研究  

Chemical constituents from dried fruits of Rubus chingii

作　　者：肖洪明[1] 祖灵博[1] 李石平[1] 王开金[1] 李宁[1] 

机构地区：[1]安徽大学生命科学学院,安徽省中药研究与开发重点实验室,安徽合肥230039

出　　处：《中国药物化学杂志》2011年第3期220-226,共7页Chinese Journal of Medicinal Chemistry

基　　金：国家自然科学基金项目(31070315);安徽省优秀青年科技基金项目(08040106812)

摘　　要：目的研究蔷薇科悬钩子属掌叶覆盆子(Rubus chingii)干燥果实的化学成分。方法采用硅胶柱色谱、Sephadex LH-20、ODS和MCI柱色谱等手段进行化合物的分离与纯化,通过波谱分析,结合理化性质鉴定各化合物的结构。结果从覆盆子体积分数80%乙醇提取物中分离得到18个已知化合物,分别鉴定为山柰酚(1)、紫云英苷(2)、山柰酚-3-O-β-D-吡喃葡萄糖醛酸甲酯(3)、山柰酚-3-O-芸香糖苷(4)、椴树苷(5)、异槲皮苷(6)、芦丁(7)、根皮苷(8)、β-谷甾醇(9)、β-胡萝卜苷(10)、乌苏酸(11)、2α,19α-dihydroxy-3-oxo-12-ursen-28-oic acid(12)、nigaichigoside F1(13)、覆盆子苷F5(14)、没食子酸(15)、短叶苏木酚酸甲酯(16)、葡萄糖(17)和甲基-β-D-吡喃葡萄糖苷(18)。结论化合物4、7、81、2、13、16和18均为首次从该植物中分离得到,其中化合物12为首次从该属植物中发现。Rubus chingii Hu（Rosaeae） is a kind of sub-shrub widely distributed in China,and the dried fruits of Rubus chingii called ＂fupenzi＂,has been used as a tonic in Traditional Chinese Medicine with the beneficial functions to liver,kidney and eyes for the treatments of kidney deficiency,erectile dysfunction,spermatorrhea,pollakisurie,enuresis,clouded vision and premature hair graying.To characterize the active constituents for the traditional effect of ＂fupenzi＂,the chemical investigation on the dried fruits of Rubus chingii was carried out.The 80% ethanol extract of the dried fruits of Rubus chingii was dissolved in H2O and partitioned with petroleum ether,EtOAc and n-BuOH sequentially to yield petroleum ether,EtOAc,n-BuOH and aqueous fraction.Eighteen compounds were isolated from the EtOAc,n-BuOH fraction by means of repeated column chromatography over silica gel,Sephadex LH-20,ODS,and MCI.Their structures were determined mainly on the basis of physicochemical and spectral data and identified as kaempferol（1）,astragalin（2）,kaempferol-3-O-β-D-glucuronic acid methyl ester（3）,kaempferol-3-O-rutinoside（4）,tiliroside（5）,isoquercitrin（6）,rutin（7）,phlorizin（8）,β-sitosterol（9）,daucosterol（10）,ursolic acid（11）,2α,19α-dihydroxy-3-oxo-12-ursen-28-oic acid（12）,nigaichigoside F1（13）,goshonoside F5（14）,gallic acid（15）,methyl brevifolin-carboxylate（16）,glucose（17） and methyl-β-D-glucopyranoside（18）.Compounds 4,7,8,12,13,16 and 18 were isolated from Rubus chingii for the first time,and compound 12 was found in genus of Rubus firstly.

关 键 词：覆盆子 悬钩子属 化学成分 

分 类 号：R284.2[医药卫生—中药学]