大孔吸附树脂分离纯化苦荞麦苗总黄酮的研究  

Study on the Purification of Total Flavonoids from Buckwheat Seedling by AB-8 Macroporous Resin

作　　者：高路[1,2] 戴建辉[2,3] 高云涛[2,3] 

机构地区：[1]云南民族大学科技处,云南昆明650031 [2]云南民族大学化学与生物技术学院,云南昆明650031 [3]国家民族事务委员会-教育部共建民族药资源化学重点实验室,云南昆明650031

出　　处：《安徽农业科学》2011年第14期8304-8306,共3页Journal of Anhui Agricultural Sciences

基　　金：云南省社会发展科技计划项目(2007B148M);民族药资源化学国家民族事务委员会-教育部共建重点实验室开放基金项目(MJY090101);云南省教育厅科学研究基金项目(2010C213)

摘　　要：[目的]为苦荞麦苗总黄酮类产品的开发和研究提供参考。[方法]研究了大孔吸附树脂分离纯化苦荞麦苗总黄酮的工艺条件。[结果]最佳工艺条件为:上样液的质量浓度为3.0 g/L,上样速率为3 BV/h,洗脱剂为80%乙醇,洗脱速率为3 BV/h,洗脱剂用量为4BV,按此工艺条件纯化后的苦荞麦苗总黄酮纯度达77.34%。[结论]AB-8型大孔吸附树脂对苦荞麦苗总黄酮有较好的吸附和解吸效果。[Objective] The aim was to provide preference for product development of total flavonoids from buckwheat seedling.[Method] The purification of total flavonoids from Buckwheat seedling by AB-8 macroporous resin was investigated.[Result]The results showed that the optimum colum conditions：the concentration and the current velocity of the original solution were 3.0 g/L,the eluant was 80%ethaol and the eluting velocity was 3.0 BV/h,the consumption of eluant was 4 BV respectively.In this condition,the purity of total flavornes was 77.34%.[Conclusion]AB-8 macroporous adsorption resin was fit for adsorbing and separating the total flavones from buckwheat seedling.

关 键 词：大孔树脂 总黄酮 纯化 苦荞麦苗 

分 类 号：S517[农业科学—作物学]