ISSR-PCR分子标记法鉴别宁杞1号与雄性不育枸杞  

Identification of No.1 and Male Sterility of Lycium barbarum L.by ISSR-PCR Molecular Marker Technique

作　　者：思彬彬[1] 王镇[1] 

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《安徽农业科学》2011年第14期8309-8310,共2页Journal of Anhui Agricultural Sciences

基　　金：北方民族大学资助项目(2007Y049)

摘　　要：[目的]探索在核酸分子水平上鉴别道地药材宁夏枸杞中不同的品种。[方法]从50条ISSR引物中筛选合适的引物对宁杞1号与雄性不育2个品种进行PCR扩增及电泳分析,寻找特征位点。[结果]1条ISSR引物扩增出较为明显的多态性特征条带,可用于宁杞1号与雄性不育的鉴别。[结论]ISSR-PCR作为一种简便、可靠的分子标记方法,可用于枸杞的鉴别。[Objective]To identify genuine medicinal materials of Lycium barbarum L.by ISSR-PCR molecular marker technique on the level of DNA molecular.[Method] Suitable primers were screened out from 50 ISSR primers（Inter-simple sequence repeat）,and then used in PCR amplification and electrophoretic analysis for distinguishing Lycium barbarum L.from No.1 and male sterility by finding specific sites.[Result]One ISSR primer could amplify polymorphic bands that were suitable for distinguishing Lycium barbarum L.from No.1 and male sterility[Conclusion] ISSR-PCR molecular marker technique was quick and reliable,which could be used in identification of Lycium barbarum L..

关 键 词：枸杞(Lycium barbarum L.) ISSR 鉴别 

分 类 号：S567.19[农业科学—中草药栽培]