不同芘处理对土壤氨氧化微生物的影响  

Effect of Different Pyrene Treatments on Soil Potential Nitrification Rate(PNR)

作　　者：刘杰[1] 彭霞薇[1,2] 

机构地区：[1]北京林业大学,北京100083 [2]中国科学院生态环境研究中心,北京100085

出　　处：《安徽农业科学》2011年第14期8398-8400,8453,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究不同芘处理对土壤氨氧化微生物的影响。[方法]采用α-萘胺显色法测定了土壤硝化潜势（PNR）,实时定量PCR方法测定了amoA基因拷贝数。[结果]芘和PNR的剂量-效应关系满足四参数的Logistic曲线,各处理土壤PNR的EC50分布在70100μg/g范围内,且随处理时间延长而增大,表明土壤微生态群落对芘产生了一定的适应或抗性;芘的急性毒性表现在中高浓度芘处理下,持久性毒性在各浓度处理下均有明显表现;芘的存在在短期内并不能改变氨氧化微生物的群落丰度及基因拷贝数。[结论]中等芘浓度范围（70100μg/g）是PNR较敏感的浓度段,芘对土壤PNR的影响可能是对氨氧化过程中关键酶AMO的竞争性抑制作用。[Objective] The aim was to study the effect of different pyrene treatments on soil potential nitrification rate.[Method] Soil PNR was detected by α-naphthylamine,and amoA gene copy number was determined by real-time quantitative PCR.[Result] The dose-response relationship between pyrene and PNR met the four-parameter logistic curve,the EC50 of soil PNR distributed among 70-100 μg/g and became larger with the expanding treatment time,indicating that the soil microbial communities had generated certain adaptation or resistance to pyrene;the acute toxicity of pyrene in the treatment appeared in the medium and high concentrations of pyrene,persistent toxic existed in all concentrations significantly;the presence of pyrene did not change the community abundance and gene copy number of ammonia-oxidation microorganism in the short term.[Conclusion] Pyrene with medium concentration（70-100 μg/g） was more sensitive to PNR,while the effects of pyrene on soil PNR might be caused by its competitive inhibition on the key enzyme during the ammonia oxidation process.

关 键 词：芘 硝化潜势 实时定量PCR 

分 类 号：S151.9[农业科学—土壤学]