新疆阿拉尔垦区猪嗜血支原体PCR检测方法的建立  

PCR Detection Method of Swine Mycoplasma haemophilus in Alar Area

作　　者：赵爱云[1] 何强[1] 井波[1] 

机构地区：[1]新疆生产建设兵团塔里木畜牧科技重点实验室塔里木大学动物科学学院,新疆阿拉尔843300

出　　处：《安徽农业科学》2011年第14期8619-8621,共3页Journal of Anhui Agricultural Sciences

基　　金：新疆生产建设兵团塔里木畜牧科技重点实验室开放课题项目(HS20902)

摘　　要：[目的]掌握阿拉尔垦区猪嗜血支原体的流行现状。[方法]根据GenBank上发表的猪嗜血支原体16S rRNA基因组序列(U88565)设计1对特异性引物,对采自新疆阿拉尔垦区的样品进行PCR扩增,并将其产物克隆到pBR322载体后测序。[结果]扩增出的片段为0.836 kb。同源性分析结果表明,该序列与猪嗜血支原体参考基因组序列同源性为99.47%,反映出我国分离株与国外株基因同源性较高。[结论]建立了一种猪嗜血支原体PCR检测方法,该方法为猪嗜血支原体病的快速诊断及流行病学调查提供了新的手段,并为相关基因的表达及功能研究奠定了基础。[Objective] The current situation of the prevalence of swine mycoplasma haemophilus in the Alar area was mastered.[Method] For the PCR amplification of the samples selected from the Alar area of Xinjiang 1 pair of specific primers was designed according to the genome sequence of 16S rRNA（U88565） of swine mycoplasma haemophilus published in GenBank and the products were sequenced after they were cloned into the pBR322 vector.[Result] The amplified fragment was 0.836 kb.The analysis showed that the homology of the sequence was 99.47% same as the reference genome sequence of swine mycoplasma haemophilus,reflecting the isolated strain of swine mycoplasma haemophilusin China was with high homologous with the strain outside.[Conclusion] The PCR detection method of swine mycoplasma haemophilus was established,which provided a new means of the rapid diagnosis of swine mycoplasma haemophilus and the investigation of epidemiology and laid the foundation in the research on the gene expression and function.

关 键 词：猪嗜血支原体 PCR 克隆 

分 类 号：S852.651[农业科学—基础兽医学]