戈米辛J对氧自由基的作用  

Effects of Gomisin J on Oxygen Free Radicals

作　　者：顾峥[1] 陈道峰[2] 胡天喜[3] 江明华[1] 

机构地区：[1]上海医科大学药学院药理学教研室,上海200032 [2]上海医科大学药学院,上海200032 [3]华东师范大学生物系,上海200062

出　　处：《上海医科大学学报》1999年第5期368-370,共3页Journal of Fudan University(Medical Science)

基　　金：国家自然科学基金!资助(392 0 0 1 64);上海市科委科技启明星项目! (95QB1 4 0 32 );国家教委跨世纪优秀人才培养计划资助

摘　　要：目的　研究戈米辛J(gomisinJ)对氧自由基的作用。方法　采用黄嘌呤/黄嘌呤氧化酶鲁米诺体系和邻苯三酚自氧化体系产生的化学发光检测超氧阴离子自由基,采用Fenton发光体系检测羟自由基。结果　戈米辛J能抑制3种体系的化学发光,对以上3种体系的IC50、IC20及IC50(95%可信限)依次分别为237.7(155.5～363.3)、100.8(88.1～115.3)和1.86(1.60～2.16)μmol/L。结论　戈米辛J具有清除氧自由基的作用。To study the effects of gomisin J on oxygen free radicals. Methods Xanthine/xanthine oxidase*.luminol chemiluminescence system,pyrogallol autoxidation chemiluminescence system,Fenton reaction phelanthroline chemiluminescence system were used to measure oxygen free radicals. Results Besides a O -· 2 scavenger with the IC 50 (95%CL) of 237.7 (155.5～363.3) μmol/L in xanthine/xanthine oxidase system and wity the IC 20 (95% CL) of 100.8 (88.1～115.3) μmol/L,gomisin J scavenged ·OH efficiently in Fenton like system,the IC 50 (95% CL) was 1.86 (1.60～2.16) μmol/L Results Besides a O -· 2 scavenger with the IC 50 (95%CL) of 237.7 (155.5～363.3) μmol/L in xanthine/xanthine oxidase system and wity the IC 20 (95% CL) of 100.8 (88.1～115.3) μmol/L,gomisin J scavenged ·OH efficiently in Fenton like system,the IC 50 (95% CL) was 1.86 (1.60～2.16) μmol/L Conclusions Gomisin J is a superoxide anion scavenger and a strong hydroxyl radical scavenger.

关 键 词：戈米辛J 自由基 化学发光 

分 类 号：R962[医药卫生—药理学]