浙江省家畜动物无形体分子流行病学调查  

Molecular epidemiological survey of Anaplasma phagtocytophilium in livestock in Zhejiang province

作　　者：陆群英[1] 张立霞[2] 孙继民[1] 姜理平[1] 柴程良[1] 郑寿贵[3] 顾时平[4] 葛君华[5] 王誓闻[2] 禹惠兰[2] 张丽娟[2] 

机构地区：[1]浙江省疾病预防控制中心微生物所,杭州310051 [2]中国疾病预防控制中心传染病预防控制所,北京102206 [3]金华市金东区疾病预防控制中心 [4]安吉县疾病预防控制中心 [5]天台县疾病预防控制中心

出　　处：《中国媒介生物学及控制杂志》2011年第3期226-228,232,共4页Chinese Journal of Vector Biology and Control

基　　金：国家基础研究项目973计划(2010CB530200;2010CB530206);重大传染病防治科技专项--细菌性传染病病原谱流行规律与变异研究(2009ZX10004-203)及病原体实验室网络化监测技术研究(2008ZX10004-008)~~

摘　　要：目的调查浙江省天台、安吉及金华县家畜动物牛及山羊无形体感染状况。方法以在册户籍为准,采用随机方法抽取调查点每农户5只山羊及5头牛,采集5ml静脉血分离血清,采用WHO推荐的微量间接免疫荧光方法(mIFA)进行血清IgG抗体检测;动物血球标本用于提取DNA进行无形体16SrRNA基因扩增。PCR产物测序并进行同源分析比较,同时进行遗传变异分析。结果当地家畜动物存在较高无形体携带率(39.42%),主要分布在天台县;无形体流行株16SrRNA基因存在较大变异性,除发现15种独立核酸变异序列外,至少存在5种优势序列变异株。结论浙江省家畜动物普遍存在无形体携带状况;进一步开展人群无形体病调查及分子流行病学研究十分必要。Objective To determine the prevalence of Anaplasma phagocytophilum in goats and oxen in Zhejiang province. Methods Five goats and five oxen were randomly surveyed in each househould based on registration information. Venous blood samples of 5 ml were collected from each subject,and the separated serum was tested for IgG antibodies against A. phagocytophilum using immunofluorescence assay（mIFA） as recommended by the WHO. The blood cells were used for DNA extraction,which was then amplified for the 16S rRNA genes of A. phagocytophilum. The PCR products were sequenced and analyzed for homology and genetic variation. Results A high carrying rate（39.42%） was found in the local livestock,particularly in Tiantai county. Considerable variation in the 16S rRNA genes was found in the prevalent strains. In addition to 15 independent variations in the nucleic acid sequences,at least five strains with variation in the dominant sequences were found. Conclusion Most domestic animals in Zhejiang province carry A. phagocytophilum warranting the survey and molecular epidemiological investigation of this pathogen in humans.

关 键 词：无形体 遗传变异 家畜动物 浙江省 

分 类 号：R376[医药卫生—病原生物学]