葫芦岛市居民区褐家鼠携带的汉坦病毒分析  

Analysis of Hantavirus carried by Rattus norvegicus in residential areas of Huludao

作　　者：李明慧[1] 陈小萍[1] 杨国庆[2] 沈铁峰[2] 刘保[2] 郭文平[1] 张永振[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206 [2]葫芦岛市疾病预防控制中心,辽宁葫芦岛125000

出　　处：《中国媒介生物学及控制杂志》2011年第3期239-242,共4页Chinese Journal of Vector Biology and Control

基　　金：国家"十五"科技攻关项目(2003BA712A08-02)~~

摘　　要：目的通过对汉坦病毒的监测分析,掌握葫芦岛市居民区鼠类中的汉坦病毒流行情况,为制定人间汉坦病毒的预防控制措施提供依据。方法用鼠笼捕获小动物,采用间接免疫荧光法(IFA)检测小动物肺中的汉坦病毒抗原,用RT?PCR进行基因分型。结果在2005-2006年选取葫芦岛地区9个不同地点的居民区和2个野外地点捕鼠,共捕获褐家鼠254只,小家鼠17只,黑线姬鼠5只,褐家鼠带病毒率为4.72%,小家鼠为5.88%。提取汉坦病毒抗原阳性鼠肺组织中的病毒RNA,从褐家鼠中共扩增出9株汉坦病毒,基因分型全部为汉城病毒,并且分离到1株病毒。而从小家鼠和黑线姬鼠中未扩增到病毒。结论葫芦岛市居民区的鼠类以褐家鼠为主,其携带的病毒属于汉城型。Objective To determine the prevalence of hantavirus in rodents based on surveillance data in residential areas of Huludao for the formulation of preventive and control strategies in humans. Methods Rodent cages were used to capture small mammals. Lung samples of the subjects were then taken for detection of Hantavirus antigens using indirect immunofluorescence assay（IFA） . Genotyping was conducted using RT-PCR. Results In 2005 and 2006,254 Rattus norvegicus,17 Mus musculus and 5 Apodemus agrarius were captured in nine residential areas and two wild fields in Huludao. The virus-carrying rates were 4.72% in R. norvegicus and 5.88% in M. musculus,respectively. Nine strains of Hantavirus were amplified using the RNA derived from the positive lung tissues of R. norvegicus. According to genotyping results,all were identified as Seoul virus（SEOV） . A strain of SEOV was also isolated. Hantavirus was not detected from M. musculus and A. agrarius. Conclusion R. norvegicus was the primary host of Hantavirus in residential areas of Huludao and all rodent-carrying Hantavirus strains were identified as SEOV.

关 键 词：汉坦病毒 褐家鼠 汉城病毒 系统发生分析 

分 类 号：S441[农业科学—农业昆虫与害虫防治] R373.32[农业科学—植物保护]