玉米秸秆同步糖化共发酵工艺条件优化  

The optimization of SSF conditions for corn stalk

作　　者：刘庆玉[1] 王晓威[1] 张大雷 

机构地区：[1]沈阳农业大学工程学院,辽宁沈阳110161 [2]辽宁省能源研究所,辽宁营口115003

出　　处：《可再生能源》2011年第3期100-103,共4页Renewable Energy Resources

基　　金：辽宁省自然科学基金(20072130)

摘　　要：以经过生物预处理后的玉米秸秆为原料,采用混合菌进行同步糖化发酵。以分光光度法测定发酵液中的乙醇含量,对发酵时间、发酵温度、接种比例、纤维素酶用量4个条件进行单因素试验分析,再通过正交试验对发酵条件进行优化。试验结果表明,最适发酵条件:发酵温度为36℃,发酵时间为96 h,接种比例为1∶1,纤维素酶用量为40 IU/g,乙醇产率为12.03%。In this paper,corn stalk,which was pretreated by biopretreatment,was used for SSF with mixed bacteria.The content of ethanol was measured by spectrophotometry,after analysis on the fermentation time,the fermentation temperature,the proportion of inoculation and the amount of cellulase separately,the fermentation conditions was optimized by orthogonal experiments.The results showed that the optimum technical condition was as follows： the temperature was 36 ℃,the fermentation time was 96 h,the proportion of inoculation was 1：1,the amount of cellulase was 40 IU/g and the yield of ethanol was 12.03%.

关 键 词：玉米秸秆 同步糖化发酵 燃料乙醇 

分 类 号：TK6[动力工程及工程热物理—生物能] S216.2[农业科学—农业机械化工程]