HPLC法测定润燥止痒胶囊中游离蒽醌的含量  

HPLC Determination of Free Anthraquinone in Runzao Zhiyang Capsule

作　　者：郑丽会[1] 夏玉吉[1] 茅向军 许乾丽 鲍家科 王珍珍 

机构地区：[1]贵州师范大学,贵州贵阳5500012 [2]贵州省药品检验所,贵州贵阳550004

出　　处：《中国药物警戒》2011年第6期327-329,共3页Chinese Journal of Pharmacovigilance

基　　金：国家药典委员会2009国家药品标准提高研究课题(项目编号:179)

摘　　要：目的建立HPLC法测定润燥止痒胶囊中何首乌、制何首乌游离蒽醌的含量。方法采用Eclipse XDB-C18(5μm,150mm×4.6mm)色谱柱,以甲醇为流动相A,0.1%磷酸溶液为流动相B,进行梯度洗脱,检测波长254nm。结果大黄素在0.0514～1.3878μg范围内线性关系良好(r=0.9999),平均回收率(n=6)为103.8%,RSD为1.1%;大黄素甲醚在0.017216～0.464832μg范围内线性关系良好(r=0.9999),平均回收率(n=6)为102.7%,RSD为1.4%。结论本法简便,准确,可作为该制剂的定量分析方法。Objective To develop an HPLC method to determine the contents of free anthraquinone in Polygonum multiflorum and Polygoni multiflori radix praeparata of Runzao Zhiyang capsule.Methods Eclipse XDB-C18（5μm,150mm×4.6mm） was adopted.Methods was used as mobile phase A and 0.1% phosphoric acid was used as mobile phase B with gradient elution.The detection wavelength was 254 nm.Results The linear range of emodin was 0.0514～1.3878μg,the recovery（n=6） of this method was 103.8% and RSD was 1.1%;The linear range of physcion was 0.017216～0.464832μg,the recovery（n=6） of this method was 102.7% and RSD was 1.4%.Conclusion The method is convenient,accurate and can be applied in quantitative determination of the drug.

关 键 词：HPLC 润燥止痒胶囊 游离蒽醌 含量测定 

分 类 号：R282[医药卫生—中药学]