鸦胆子不同组分抗炎与伴随毒副作用研究  

Study on Anti-inflammatory Effect and Adjoint Toxical and Side Effects of Different Extract from Fructus Bruceae

作　　者：杨倩[1,2] 吕莉莉[1] 张丽美[1] 孙蓉[1] 

机构地区：[1]山东省中医药研究院,山东济南2500142 [2]山东中医药大学,山东济南250355

出　　处：《中国药物警戒》2011年第6期336-338,共3页Chinese Journal of Pharmacovigilance

基　　金：国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)

摘　　要：目的对鸦胆子水提、醇提组分发挥抗炎药效剂量下出现的伴随毒副作用进行观察与研究,为阐明其"毒性-功效"相关性提供实验依据。方法建立小鼠巴豆油耳肿胀模型和琼脂肉芽肿模型,并分别给小鼠灌胃高、中、低不同剂量的鸦胆子水提、醇提组分连续3天和7天,末次给药后取血检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性及肌酐(Cr)、尿素氮(BUN)含量变化并取肝、肾称重计算脏体比值。结果连续多次给小鼠灌胃药效剂量的鸦胆子水提和醇提组分均可明显抑制巴豆油致小鼠耳肿胀和琼脂致小鼠肉芽肿,且水提组分的肿胀抑制率高于醇提组分,且呈现良好的量-效关系;给药后小鼠血清ALT、AST活性及肝体比值均无明显变化,Cr、BUN含量及肾体比值升高,醇提组分的升高幅度大于水提组分,"量-毒"、"时-毒"关系较明显。结论药效剂量的鸦胆子水提、醇提组分对急慢性炎症均有良好的抗炎作用,同时可对肾脏产生一定的伴随毒副作用,其毒副作用机制还有待进一步研究。Objective To offer experimental basement for the elucidation of dependablity between ＂toxicity-efficacy＂ by researching the adjoint toxical and side effects of anti-inflammatory effect of water and alcohol extract from Fructus Bruceae.Methods The model of ear swelling by croton oil and granuloma by agar were made and model mice were administrated with water and alcohol extract from Fructus Bruceae of high,middle and low dosage 3/7 days continually.The activities of serum ALT,AST and contents of Cr,BUN were detected while the ratio of liver and kidney to body was measured.Results Both ear swelling by croton oil and granuloma by agar can be inhibited by multiple intragastric administration of water and alcohol extract from Fructus Bruceae.The swelling depressive rate of water extract was higher than that of alcohol extract and showed a well ＂dose-effect＂ relationship.The activities of serum ALT,AST and ratio of liver to body showed little change,but both the contents of Cr and BUN and ratio of kidney to body increased significantly with doses and time.Conclusion Both water and alcohol extract from Fructus Bruceae at effective dosage could efficiently inhibit acute and chronic inflammation.At the same time some toxical and side effects can be caused to kidney,however,the occurrence mechanism of its toxical and side effects still need further study.

关 键 词：鸦胆子 抗炎 伴随毒副作用 

分 类 号：R282[医药卫生—中药学]