银杏内酯对过氧化氢损伤的皮层神经元和SH-SY5Y细胞亲环蛋白A(CypA)表达的影响  

Effect of Ginkgolides on the expression of CypA in primary cultured neurons and SH-SY5Y cells injuried by H_2O_2

作　　者：杨俊霞[1] 杨惠超 王东江 潘志强[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室&江苏省麻醉与镇痛应用技术重点实验室,江苏徐州221000 [2]北京金康驰医药投资有限公司,北京100142 [3]河北省临漳县人民医院胸外科,河北临漳056600

出　　处：《中国医药导报》2011年第17期5-8,共4页China Medical Herald

摘　　要：目的:研究银杏内酯(ginkgolides,Gin)对过氧化氢(H2O2)诱导的皮层神经元及SH-SY5Y细胞(神经母细胞瘤细胞)损伤的保护作用及其机制。方法:在培养1周的小鼠皮层神经元培养基中加入H2O2,建立神经元氧化损伤模型,用同样方法建立SH-SY5Y细胞氧化损伤模型。采用MTT比色法检测细胞活力,Western Blot技术分析亲环蛋白A的表达变化。结果:H2O2可诱导神经元和SH-SY5Y细胞损伤,使细胞活力降低,可下调亲环蛋白A的表达;Gin预处理12 h可提高细胞活力,可使亲环蛋白A表达增加。结论:Gin可抑制H2O2引起的神经元及SH-SY5Y细胞损伤,该保护作用与其上调亲环蛋白A的表达有关。Objective： To study the protective effects of ginkgolides（Gin） in primary cultured neurons and SH-SY5Y cells injuried by H2O2 and its action mechanism.Methods： Oxidative injury model of neurons and SH-SY5Y cells was induced by H2O2 in mice.The cell activity was detected by MTT,meanwhile the expression of CypA was observed by Western Blot.Results： H2O2 could both induce oxidative injury in neurons and SH-SY5Y cells,decreased the cell activity,inhibited the expression of CypA.Besides ginkgolides preconditioning could both up-regulate the cell activity and the expression of CypA.Conclusion： Ginkgolides could inhibit the injury in primary cultured neurons and SH-SY5Y cells injuried by H2O2.This protective effect may be associated with the up-regulation of the expression of CypA.

关 键 词：银杏内酯 过氧化氢 亲环蛋白A 神经元 SH-SY5Y细胞 

分 类 号：R96[医药卫生—药理学]