胰腺大部切除法制作大鼠高血糖模型的实验研究  

Study on hyperglycemia model of rats induced by subtotal pancreatectomy

作　　者：李勇[1] 章乐虹[2] 

机构地区：[1]四川省雅安市人民医院普外科,四川雅安625000 [2]广州医学院第二附属医院普外科,广东广州510260

出　　处：《中国医药导报》2011年第17期37-40,共4页China Medical Herald

基　　金：广东省广州市高校科技计划项目(1033)

摘　　要：目的:使用胰腺大部切除法制作大鼠高血糖动物模型。方法:60只SD大鼠分为三组,P+H组(n=30):切除大鼠胰腺>90%,后予高糖饮食刺激。P组(n=20):只行胰腺大部切除。H组(n=10):假手术加高糖饮食。观察术后大鼠血糖、胰岛素、体重变化及糖耐量反应并对胰腺组织切片行HE及免疫组化染色检查。结果:术后24 h起多数实验动物出现血糖升高,但未出现典型的糖尿病表现,术后2个月,部分血糖持续升高>7 mmol/L大鼠胰岛形态学检查可见有不同程度的胰岛萎缩、beta细胞数量减少,而其他大鼠则可见胰岛肥大,胰岛素染色增强。不予高糖饮食刺激组在出现一过性血糖升高后又渐回复正常,但糖耐量反应仍出现异常。结论:切除大鼠大部胰腺后,予高糖饮食刺激可制作稳定的高血糖动物模型,其中适当轻度血糖升高可能有助于促进胰岛再生,而持续较高的血糖水平则可加重胰腺损伤时胰岛负担并致其损伤。Objective： To get hyperglycemia model of rats by subtotal pancreatectomy.Methods： Sixty rats were divided into three teams,team P＋H（n=30）：resect more than 90% of pancreas,then the rats were given high-carbohydrate diet.Team P（n=20）：only receive subtotal pancreatectomy.Team H（n=10）：receive false surgery and high-carbohydrate diet.Changes of blood sugar,insulin,weight and glucose tolerance reaction were monitored,the tissue section of remnant pancreas were stained by HE and immunohistochemistry.Results： 24 hours after operation,blood sugar of most rats were rising,but there was no typical diabetic symptom observed.Morphological investigation on pancreas showed the deduction and atrophy of islet to varying degree after 2 month of operation in some of the rats which blood sugar constantly more than 7 mol/L,while the others showed hypertrophy and enhancement of insulin stain in the islets.In the normal diet team,blood sugar gradually decreased after 1-3 d increasing,but the sugar-resist test still showed glucose intolerance.Conclusion： Stable hyperglycemia model of rats can be made by pancreatectomy and high-carbohydrate diet.Moderate hyperglycemia may be contribute to the regeneration of islet,while long-term over increase of blood sugar may cause over loading and injury of beta-cells.

关 键 词：高血糖 胰腺大部切除 动物模型 

分 类 号：R965.1[医药卫生—药理学]