丝状噬菌体随机呈现肽库的构建与鉴定  

Construction and identification of a random peptide library with displaying filamentaous phage

作　　者：万瑛[1] 代佳平[1] 王燕[1] 刘昕[1] 

机构地区：[1]第三军医大学基础医学部分子生物学教研室,重庆400038

出　　处：《第三军医大学学报》1999年第11期837-839,共3页Journal of Third Military Medical University

摘　　要：目的：构建一个随机２０个氨基酸的丝状噬菌体肽库。方法：扩增随机合成ＤＮＡ模板，经限制性内切酶ＸｈｏⅠ＋ ＳｐｅⅠ酶切后克隆进噬菌粒ｐＴＭＢ２，构建随机丝状噬菌体肽库并检验其库容及随机性。结果：完成丝状噬菌体随机肽库的构建，其库容为２．１×１０８，其氨基酸分布于理论频数无显著差异。结论：丝状噬菌体随机肽库被成功构建。Objective: To construct a library of M13 PIII fusion displaying peptides composed of 20 random amino acids. Methods: Random oligonucleotides encoded with 20 peptides were amplified and the amplified products after digested with Xho Ⅰ and Spe Ⅰ were cloned into plasmid pTMB2 to construct the library. Then the number of different recombinants and the randomness of the library were identified. Results: A random peptide library with 2.1×10 8 different recombinant clones was obtained. No significant differnece was found between te amino acid distribution of the peptide library and the expected frequency. Conclusion: The random peptide library has been successfully constructed.

关 键 词：丝状噬菌体 随机肽库 pTMB2 构建 鉴定 

分 类 号：Q78[生物学—分子生物学] Q939.48