免疫酶斑点法(IEDA)检测白色念珠菌芽管特异性抗原的临床研究  

Clinical observation of detecting germ tube specific antigen of Candid alblicans by IEDA

作　　者：谢卫红[1] 叶庆佾[1] 廖兴元 

机构地区：[1]第三军医大学附属西南医院皮肤科,重庆400038

出　　处：《第三军医大学学报》1999年第11期846-848,共3页Journal of Third Military Medical University

摘　　要：目的：试图寻求一种较理想的系统性念珠菌病的血清学诊断方法。方法：制备白色念珠菌芽管特异性抗体，采用ＩＥＤＡ对临床病人血清中的相应抗原进行检测。结果：①检测的最低敏感浓度为０．２５ μｇ／ｍ ｌ；②以１４ 例系统性念珠菌病患者为实验组，以１５ 例浅表白念感染者，１１ 例其它疾病患者及２０位正常人为对照组，检测的敏感性为７１．４％ ，特异性为１００％ 。结论：该方法敏感性较高，特异性强，且具有简便经济的特点。有助于系统性念珠菌病的诊断，尤其适宜在基层医院普及应用。Objective: To find a better serologic diagnostic method for systemic candidiasis. Methods: Germ tube specific antibody of Candida albicans was prepared and used to detect corresponding antigen in serum of patients by IEDA. Results: ①The lowest sensitive concentration was 0.25 ng/ml; ②The sensitivity of this method was 71.4%. There was no germ tube specific antigen detected in sera of 15 patients with superficial candidiasis, 11 with other diseases and 20 normal controls. The specificity was 100%. Conclusion: This method has high sensitivity and the method is simple. So IEDA can be helpful to the diagnosis of systemic candidiasis.

关 键 词：白色念球菌 芽管 特异性抗原 免疫酶斑点法 

分 类 号：R519.304[医药卫生—内科学]