薄层扫描法测定通脉活络丸中黄芪甲苷的含量  

Determination of astragaloside A in Tongmai Huoluo Pills by TLC scanning

作　　者：韩建伟[1] 芦喜珍[1] 熊丽 余正友 

机构地区：[1]湖北中医学院,武汉430061 [2]湖北省十堰市药检所 [3]襄樊市药材供应站

出　　处：《中国医院药学杂志》1999年第11期667-668,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的：测定通脉活络丸中黄芪甲苷的含量。方法：用甲醇边提取边皂化，然后用氯仿正丁醇（２∶１） 萃取等步骤制成供试品，以薄层扫描法测定，λＳ＝５３０ ｎｍ ，λＲ＝ ６８０ ｎｍ 。结果：平均回收率为９７．４７％ ，ＲＳＤ＝２ ．４２ ％ ，两批样品中黄芪甲苷的含量分别为０ ．０３８ ９ ％ 和０．０４１ ５％ 。结论：该方法较为简便，经济。OBJECTIVE:To determine astragaloside A in Tongmai Huoluo pills.METHODS: Tongmai Huoluo pills were extracted and saponificated with methyl alcohol simultaneously,and then were extracted and processed into the samples with chloroform butanal(2∶1) and so on,finally the samples were determinated by TLC scanning(λ S=530 nm,λ R=680 nm).RESULTS:Average recovery was 97.47% ,and RSD = 2.42% .The contents of the astragaloside A in two groups of samples are 0.038 9% and 0.041 5% respectively.CONCLUSIONS:The method is simple,convenient and economical.

关 键 词：薄层扫描法 通脉活络丸 含量测定 黄芪甲苷 

分 类 号：R286.0[医药卫生—中药学]