饲料磷脂水平对巴丁鱼(Pangasius sutchi)鳃Na^+-K^+-ATPase活性及细胞膜脂肪酸组成的影响  

Effects of dietary phospholipid on Na^+-K^+-ATPase activities and cell membrane fatty acid composition in gill of catfish(Pangasius sutchi)

作　　者：麻艳群[1,2] 黄凯[1] 于丹[1] 陈涛[1] 卢克焕[1,2] 

机构地区：[1]广西大学动物科技学院,南宁530004 [2]广西亚热带生物资源保护利用重点实验室,南宁530004

出　　处：《安徽农业大学学报》2011年第3期346-351,共6页Journal of Anhui Agricultural University

基　　金：广西大学科研基金项目(XGL090011);广西自然科学基金项目(桂科自0728022)共同资助

摘　　要：试验旨在研究饲料磷脂水平对巴丁鱼(Pangasius sutchi)鳃中的Na+-K+-ATPase活性及细胞膜脂肪酸组成的影响。对巴丁鱼(初始体重约1.45±0.08 g.尾-1)分别投喂5组饲料,各组饲料磷脂添加水平为0%(PL0组)、1%(PL1组)、2%(PL2组)、3%(PL3组)和4%(PL4组),饲养56 d。结果显示,鱼鳃中的Na+-K+-ATPase活性随着饲料磷脂水平的升高而下降(P<0.05);鳃膜脂肪酸组成发生显著变化:饱和脂肪酸(saturated fatty acids,SFA)含量以对照组(PL0组)的最高,与PL2、PL3组差异显著(P<0.05);PL0组的单不饱和脂肪酸(monounsaturated fatty acids,MUFA)含量显著高于其余4组(P<0.05);PL3组的多不饱和脂肪酸(polyunsaturated fatty acids,PUFA)含量最高,与PL0、PL1组有显著差异(P<0.05);PL0组的高不饱和脂肪酸(highly unsaturated fatty acids,HUFA)含量显著低于其他添加了磷脂的各试验组(P<0.05)。结果表明,饲料磷脂水平对巴丁鱼鳃Na+-K+-ATPase活性有一定影响,未添加磷脂的饲养条件下鳃Na+-K+-ATPase表现出较强的补偿能力,以维持稳定的生理水平及正常的基础代谢;巴丁鱼鳃细胞膜中的∑HUFA和∑PUFA含量显著高于对照组,有助于细胞膜更好地执行和完成正常生理功能。The aim of this study was to determine the effects of dietary phospholipid on Na＋-K＋-ATPase ac-tivities and cell membrane fatty acid composition in the gill of catfish（Pangasius sutchi）.900 healthy catfish（1.4±0.08g of average weight） were randomly divided into five groups.Dietary phospholipid level of group PL0 was 0%,and 1%,2%,3%,4% for group PL1,group PL2,group PL3,group PL4,respectively.The experiment lasted for 56 days.The results showed that the Na＋-K＋-ATPase activities in gill decreased gradually with the in-crease of the dietary phospholipid level（P〈0.05）.Cell membrane fatty acid composition was affected significantly by dietary phospholipids.The content of saturated fatty acids and monounsaturated fatty acids of group PL0 was highest（P〈0.05）,however,it had the lowest content of highly unsaturated fatty acids（P〈0.05）.The content of polyunsaturated fatty acids in group 3 was higher than that in group 0 and group 1（P〈 0.05）.The results indicate that there is obvious compensation effect on Na＋-K＋-ATPase activity with phospholipids content changes in gills of catfish（Pangasius sutchi） and the increase of ∑HUFA and ∑PUFA is necessary for gill cell membrane to maintain and perform normal physiological function

关 键 词：磷脂 巴丁鱼 NA^+-K^+-ATPASE 膜脂肪酸 

分 类 号：S965.199[农业科学—水产养殖]