棉花盐胁迫应答基因GhCPK5的克隆及序列分析  

Isolation and sequence analysis of salt responsive gene GhCPK5 from Gossypium hirsutum L.

作　　者：甄军波[1] 张曦[1] 王玉美[2] 翟志席[1] 李召虎[1] 华金平[1] 

机构地区：[1]中国农业大学农学与生物技术学院/农业部作物基因组学与遗传改良重点开放实验室/作物改良北京市重点实验室,北京100193 [2]湖北省农业科学院经济作物研究所,武汉430064

出　　处：《中国农业大学学报》2011年第3期8-14,共7页Journal of China Agricultural University

基　　金：国家"863"计划项目(2006AA10A108);教育部新世纪优秀人才支持计划项目(NCET-06-0106)

摘　　要：本研究克隆了1个陆地棉新的钙依赖蛋白激酶基因GhCPK5(Genbank登录号为:HM002634)。GhCPK5基因组DNA序列全长3 031 bp,包含一个1 707 bp的开放阅读框,具有7个外显子和6个内含子。GhCPK5可能定位于细胞核内,有568个氨基酸残基,分子量为63 ku。氨基酸序列分析表明,陆地棉GhCPK5与杨树PtCPK5同源性达到89%,同样包含完整的激酶结构域和4个EF-hand结构域。RT-PCR分析表明,该基因在根、茎、叶等组织中表达;在盐胁迫处理下,GhCPK5在各组织的表达量明显上升。In this study,a salt responsive gene GhCPK5was firstly obtained from upland cotton using RT-PCR and RACE.GhCPK5located probably in cell nucleus and the genomic sequence indicated that GhCPK5had 7 exons and 6 introns.GhCPK5contained an open reading frame（ORF） of 1 707 bp,which encoded a protein with 568 amino acids,with a molecular weight of 63 ku.GhCPK5 contained one protein kinase domain and four conserved EF-hand domains.The amino acid sequence of GhCPK5 shared 89% identify with Populus trichocarpa homolog PtCPK5.RT-PCR analysis revealed that GhCPK5expressed in root,stem and leaf,and its expression level was significantly up-regulated by salt stress.

关 键 词：棉花 GhCPK5 盐胁迫应答 RACE 序列分析 

分 类 号：S562[农业科学—作物学] Q785[生物学—分子生物学]