骨髓间充质干细胞移植对VCD所致卵巢损伤的修复研究  

Bone Marrow Mesenchymal Stem Cell Transplantation for Premature Ovarian Failure Induced by 4-vinylcyclohexene Diepoxide

作　　者：王琰[1] 杨瑛[2] 刘兵 陈宥艺[1] 陈冬波 

机构地区：[1]第四军医大学唐都医院妇产科,陕西西安710038 [2]解放军307医院妇产科,北京100071 [3]解放军307医院肿瘤学研究室,北京100071

出　　处：《现代生物医学进展》2011年第10期1844-1846,1850,共4页Progress in Modern Biomedicine

基　　金：国家"863"计划资助子课题(2008AA8070099)

摘　　要：目的:探讨小鼠骨髓间充质干细胞(MSCs)移植对去氧乙烯基环己烯(VCD)所致卵巢早衰治疗的可行性。方法:采用VCD(160mg kg-1,day-1)连续腹腔注射来诱导小鼠卵巢早衰。每侧卵巢注射转染了绿色荧光基因小鼠骨髓来源的MSCs,于移植后14、28天及45天,取各组血液标本及卵巢组织,同时观察小鼠动情周期的变化;酶联免疫法检测血清FSH、LH水平,显微镜下观察MSC在卵巢的分布。结果:MSCs移植后各组均可见绿色荧光,并且主要分布于卵巢间质区,卵巢泡膜细胞区也可见绿色荧光细胞。MSCs组动情周期较实验对照组缩短,FSH与LH水平较实验对照组低,差异具有显著性。结论:骨髓间充质干细胞可改善卵巢早衰小鼠的卵巢内分泌功能,并且长时间存在于卵巢组织。骨髓间充质干细胞可能成为卵巢早衰治疗的新方法。To investigate the therapeutic potency of MSCs transplantation on premature ovarian failure induced by 4-vinylcyclohexene diepoxide （VCD）.Methods： Female C57 mice were dosed with VCD （160mg kg^-1 day^-1, ip） daily to induce ovarian failure. GFP-labeled BMSCs were injected into the ovary. The FSH and LH in the serum were detected and the ovaries were collected at 14d, 28d and 45d after transpantatipn. GFP-labeled MSCs were tracked in the ovaries. Ovarian follicle populations were also determined. Results： There were fluorescent cells in ovarian tissues of every time after transplantation. Estrous cycles were shorten in the transplantation group than that in the model group. The FSH and LH in transplantation group at 14, 28, 45days after transplantation were significantly lower than those in the model group. Conclusions： The transplantation MSCs can improve the ovary function which damaged by VCD. MSCs can migrate in the mouse ovaries and exist in ovarian tissues of mouse for a long time. MSCs may be a new treatment for premature ovary failure.

关 键 词：骨髓间充质干细胞 VCD 卵巢早衰 模型 小鼠 

分 类 号：R711.75[医药卫生—妇产科学]