红毛五加皮的HPLC指纹图谱研究  

HPLC fingerprint of Acanthopanacis giraldii Cortex

作　　者：钟世红[1] 卫莹芳[2] 古锐[2] 熊玲娟[1] 

机构地区：[1]成都医学院药学院,四川成都610083 [2]成都中医药大学,四川成都611131

出　　处：《中成药》2011年第5期737-741,共5页Chinese Traditional Patent Medicine

摘　　要：目的建立红毛五加皮的HPLC指纹图谱。方法 Welchrom C18色谱柱,乙腈-0.3%磷酸二氢钠水溶液梯度洗脱系统(加磷酸调节pH值约为3.5),检测波长207 nm;柱温35°C。结果对47批红毛五加皮指纹图谱进行评价,确定了17个共有峰,指认了第8、9、13号色谱峰分别对应为绿原酸、刺五加苷B和刺五加苷E,其中刺五加苷E为第一大色谱峰。从峰面积比较,林窗样品总峰面积大于林下样品;两年生茎皮中刺五加苷E峰面积远大于一年生样品。结论本研究可为红毛五加皮质量控制提供参考。日照有利于红毛五加皮中成分的积累,刺五加苷E在茎皮生长的第二年积累迅速。AIM To establish an HPLC fingerprint of Acanthopanacis giraldii Cortex.METHODS Separation was performed on Welchrom-C18 chromatographic column,acetonitrile-0.3% sodium dihydrogen phosphate as mobile phase with gradient elution.The detection wavelength was set at 207 nm,the column temperature was 35 °C.RESULTS Seventeen common chromatographic peaks were set up among forty-seven batches of Acanthopanacis giraldii Cortex.The identified peaks No.8,9,13 were chlorogenic acid,eleutheroside B and eleutheroside E,respectively.The peak of eleutheroside E was the greatest one in the fingerprint chromatogram.The total peaks area of the samples from the forest gap were larger than those from the wood land,and the peak area of eleutheroside E of biennial samples was far away larger than those of annual samples.CONCLUSION The study can provide reference material for the quality control of Acanthopanacis giraldii Cortex.Sunshine is good for its component accumulation,the amount of eleutheroside E accumulates quickly in stem bark during the growth of the second year.

关 键 词：红毛五加皮 指纹图谱 HPLC 刺五加苷E 绿原酸 

分 类 号：R284.1[医药卫生—中药学]