去痴灵对阿尔茨海默病模型动物的预防性作用  

Preventive effect of Quchiling on Alzheimer's disease mouse model

作　　者：赖永长[1] 侯军代[1] 张晓裕[1] 罗焕敏[1,2,3] 

机构地区：[1]暨南大学医学院药理学系 [2]暨南大学神经科学研究所 [3]暨南大学-香港大学脑功能与健康联合实验室,广东广州510632

出　　处：《中成药》2011年第5期757-761,共5页Chinese Traditional Patent Medicine

基　　金：国家"211工程"三期建设基金资助(2005-2010);广东省科技计划项目(2010B030700018)

摘　　要：目的探讨去痴灵(广东海风藤、五味子、石菖蒲等)对阿尔茨海默病(AD)动物模型的预防作用。方法昆明种小鼠经皮下注射D-半乳糖120 mg/(kg·d)和灌胃三氯化铝20 mg/(kg·d)联合造模10周;造模第7周开始,试药组同时给予低、中、高剂量去痴灵分别为2.3 g/(kg·d)、4.6 g/(kg·d)、9.2 g/(kg·d)至第10周;通过Morris水迷宫和跳台测试小鼠学习记忆,RT-PCR测定β-淀粉样蛋白前体(APP)mRNA表达;采用HE染色、Bielschowsky改良法染色及Aβ免疫组化染色观察小鼠脑组织形态学变化。结果与模型对照组比较,去痴灵各剂量组小鼠逃避潜伏期缩短、平台所在象限时间百分比和穿越平台次数增加(P<0.05),跳台错误次数明显减少、APP mRNA相对含量明显降低(P<0.01),海马神经元排列整齐,结构完整,染色均匀,胞质与胞核清晰可辨,神经原纤维缠结和老年斑数目明显减少,无变性样斑块。结论去痴灵对D-半乳糖和三氯化铝联合制备的AD模型具有一定的预防作用。AIM To investigate the preventive effect of Quchiling（Kadsura heteroclita（Roxb） craib,Sehisandrae chinesis Fructus,Acorus tatarinowii Schott,etc） on Alzheimer＇s disease mouse model.METHODS The mice were treated with intraperitoneal administration of D-galactose 120 mg/（kg·d） and intragastric administration of AlCl3 20 mg/（kg·d） for 10 weeks;the therapy groups administrated Quchiling with concentration of（2.3 g/（kg·d）,4.6 g/（kg·d）,9.2 g/（kg·d）） from the 7th week to the 10th week.The learning and memory abilities of mice were assessed by the performance in Morris water maze and step down test.The expression of amyloid beta-protein precursor（APP） was analyzed by RT-PCR.Hematoxylin-eosin（HE） staining,neurofibrillary tangles（NFT） staining and amyloid beta protein（Aβ） immunohistochemistry were applied to detecting the pathological changes in brain.RESULTS As compared with model group,in Quchiling-treated groups,the escape latency was shortened;the number of times that mice crossed the original platform location and the percentage of swimming time spent in the target quadrant increased（P0.05）;the error frequency of step down test and the expression of APPmRNA significantly decreased（P0.01）;the neurons in hippocampus were intact and arranged regularly and the dyeing was uniform and stable;the number of neurofibrillary tangles and senile plaque was decreased.CONCLUSION Quchiling could prevent the mouse model of Alzheimer＇s disease induced by combination of D-galactose and Aluminum trichloride.

关 键 词：去痴灵 阿尔茨海默病 学习记忆 Β-淀粉样蛋白 小鼠 

分 类 号：R285.5[医药卫生—中药学]