姜黄素-PLGA纳米粒的制备与药效学评价  

Preparation and pharmacodynamic evaluation of curcumine-PLGA nanoparticles

作　　者：朱志新[1] 钱颖[1] 曹青日[1] 杨世林[1] 崔京浩[1] 

机构地区：[1]苏州大学医学部药学院,江苏苏州215123

出　　处：《中成药》2011年第5期776-779,共4页Chinese Traditional Patent Medicine

基　　金：科技部国际科技合作项目(2009DFA31330);国家"重大新药创制"科技重大专项(2009ZX09502-009)

摘　　要：目的制备和考察姜黄素-聚乳酸/羟基乙酸纳米粒(Cur-PLGA NPs)对小鼠溃疡性结肠炎的影响。方法乳化-溶剂挥发法制备Cur-PLGA NPs,透射电镜观察外观形态,动态激光粒度分析仪分析微粒大小及分布,测定载药量。通过自由饮用5%硫酸葡聚糖钠(DSS)溶液7 d,诱导小鼠溃疡性结肠炎,考察Cur-PLGA NPs对模型动物疾病活动指数(DAI)的影响。结果姜黄素PLGA纳米粒的平均大小为(419±12)nm,载药量为(15.8±1.0)%。小鼠DAI(第7天)从小到大顺序依次为:正常组<纳米粒高剂量组<5-氨基水杨酸组<混悬液组<纳米粒中剂量组<纳米粒低剂量组<阴性对照组。结论姜黄素PLGA纳米粒对溃疡性结肠炎小鼠具有较好防治作用。AIM To prepare and investigate curcumin loaded PLGA nanoparticles（Cur-PLGA NPs） on the ulcerative colitis（UC） in mouse.METHODS Cur-PLGA-NPs were prepared by modified emulsion-solvent evaporation method.The shape and size of Cur-PLGA-NPs were observed by TEM and dynamic laser scattering analyzer,respectively.Loading capacity of drug in nanoparticles was determined.UC model mice were induced by oral administration of 5% sodium dextran sulfate（DSS） for 7 days,the pharmacodynamic effect of Cur-PLGA NPs was investigated in terms of disease active index（DAI）.RESULTS The average particle size and drug loading of nanoparticles were（419±12） nm and（15.8±1.0）%,respectively.The order of DAI on the 7th day from low to high was as follows,normal Cur-PLGA NPs-High dose 5-ASA Cur suspension Cur-PLGA NPs-Middle dose Cur-PLGA NPs-Low dose control group.CONCLUSION Curcumine-PLGA nanoparticles displayed therapeutic and prevent effect on UC in mouse.

关 键 词：姜黄素 PLGA 纳米粒 溃疡性结肠炎 疾病活动指数 小鼠 

分 类 号：R944[医药卫生—药剂学]