不同相对截留分子量聚醚砜超滤膜精制黄连解毒汤效果的研究  

Study on refining Huanlian Jiedu Decoction with different relative molecular weight cutoff polyethersulfone ultraffiltration membranes

作　　者：须明玉[1] 彭稳稳[1] 钱智磊[1] 董洁[1] 郭立玮[1] 

机构地区：[1]南京中医药大学中药复方分离工程重点实验室,江苏南京210029

出　　处：《中成药》2011年第5期779-783,共5页Chinese Traditional Patent Medicine

基　　金：南京中医药大学基础研究项目(08XYC05);国家自然科学基金项目(30873450);江苏省教育厅青蓝工程资助项目

摘　　要：目的以黄连解毒汤(黄连、黄柏、黄芩和栀子)为研究对象,探讨采用不同相对截留分子量聚醚砜超滤膜处理后高分子物质和指标性成分变化规律以及药理作用差异,为优选黄连解毒汤超滤工艺研究提供依据。方法逐级用pes-5w、pes-3w、pes-2w、pes-6k的超滤膜处理黄连解毒汤离心液,测定不同膜处理后渗透液和离心液中高分子物质(果胶和蛋白质)和药用指标性成分(栀子苷、黄芩苷、巴马汀和小檗碱)的质量分数及其对嗜铬细胞瘤(PC-12细胞)缺血缺氧的保护和改善作用。结果随着膜相对截留分子量的降低,各级渗透液的高分子物质的质量分数不同程度地降低;指标性成分的质量分数除黄芩苷逐级降低外,其他都呈升高趋势;pes-3w的综合指标性成分质量分数最高;药理实验证明pes-5w渗透液对PC-12细胞缺氧缺血有很好的改善作用。结论 pes-5w超滤膜既能有效去除非药用成分或药用性较差的物质,又能保留有效成分,适合于黄连解毒汤的精制。AIM To study the effect of polyethersulfone ultraffiltration membranes with relative entrapping molecular weights on the refinement of Huanlian Jiedu Decoction（Coptidis Rhizome,Phellodendri chinensis Cortex,Scutellariae Radix and Gardeniae Fructus）.METHODS The centrifugated extract of Huanglian Jiedu Decoction was tackled by different membranes including pes-5w,pes-3w,pes-2w,pes-6k in turn.The contents of polymeride matter（pectin and protein） and medicinal ingredients（gardoside,scutellarin,palmatine and berberine） were used as markers.Besides,the protective effect against ischemia hypoxia was carried out on pheochromocytoma（PC12 cell line）.RESULTS With the relative entrapping molecular weight membrane decreasing,the contents of polymeride decreased gradually.Meanwhile,the contents of medicinal ingredients increased except baicalin content and the filtrate of pes-3w was one of the best membrane for comprehensive analysis.The experiment of in vitro PC-12 cell showed that pes-5w had better protective effect against ischemia and hypoxia.CONCLUSION Ultrafiltration membrane of pes-5w can not only remove non-medicinal ingredients effectively,but also retain the active ingredients and proper for refining Huanglian Jiedu Decoction.

关 键 词：黄连解毒汤 超滤 高分子物质 指标性成分 PC12细胞 

分 类 号：R284.2[医药卫生—中药学]