乌药总生物碱的大鼠在体肠吸收研究  

In situ intestinal absorption of total alkaloids from Linderae Radix in rats

作　　者：谭蓉[1] 侴桂新[1,2] 冯年平[1] 赵继会[1] 王志[1] 

机构地区：[1]上海中医药大学,上海201203 [2]上海中药标准化研究中心,上海201203

出　　处：《中成药》2011年第5期787-790,共4页Chinese Traditional Patent Medicine

基　　金：国家科技部"重大新药创制"科技重大专项(2009ZX09103-374);教育部新世纪优秀人才支持计划(NECT08-0898)

摘　　要：目的研究乌药总生物碱的大鼠在体肠吸收情况,为口服剂型设计提供理论依据。方法采用大鼠在体单向肠灌流实验,以去甲异波尔定做为指标性成分,采用HPLC法测定吸收部位、灌肠液中乌药总生物碱质量浓度,考察灌肠液的pH值对乌药总生物碱吸收的影响。结果乌药总生物碱在各肠段吸收较好;不同质量浓度的吸收速率常数(ka)和表观吸收系数(Papp)无显著性差异(P>0.05);灌流液不同pH值的ka和Papp值有显著性差异(P<0.05)。结论乌药总生物碱在全肠段吸收较好,不受其质量浓度的影响,提示其吸收机制主要为被动扩散吸收。AIM To study the in situ intestinal absorption of total spicebush alkaloids（TSA） from Linderae Radix in rats.METHODS TSA concentration in rat intestinal segment was determined by HPLC with norisoboldine as the marker.Unidirectional enteroclysm was employed to investigate the interaction between intestinal segments,TSA content concentration and pH of TSA on absorption rate constant（ka） of TSA and the intestinal apparent permeation coefficients（Papp）.RESULTS TSA was well absorbed at all segments of intestine in rats.The values of the ka and the Papp were significantly affected by pH of perfusion solution and but for the concentration of TSA.CONCLUSION TSA is well absorbed at all segments of intestine in rat,suggesting passive diffusion mechanism might be involved in the absorption of TSA.

关 键 词：在体肠吸收 乌药总生物碱 单向灌流法 高效液相色谱法 

分 类 号：R966[医药卫生—药理学]