地榆提取物中不同类型鞣质的测定  

Determination of tannins content in Sanguisorba officinalis L. extracts

作　　者：程悦[1] 陈嘉升[1] 陈建萍[2] 王冬梅[1] 

机构地区：[1]中山大学药学院,广东广州510006 [2]香港大学中医药学院

出　　处：《中成药》2011年第5期852-857,共6页Chinese Traditional Patent Medicine

摘　　要：目的建立可水解鞣质和缩合鞣质测定方法,并对地榆水提物及不同极性溶剂萃取物中可水解鞣质和缩合鞣质进行测定。方法采用酸解后HPLC分析法测定可水解鞣质;采用香草醛硫酸法测定缩合鞣质。结果地榆水提物、乙酸乙酯萃取物、正丁醇萃取物、水层留余物的可水解鞣质质量分数分别为(81.83±2.59)mg/g、(206.80±2.43)mg/g、(163.56±5.69)mg/g、(47.62±1.13)mg/g;缩合鞣质质量分数分别为(181.72±1.41)mg/g、(594.52±16.76)mg/g、(476.27±13.33)mg/g、(14.41±0.29)mg/g。结论酸解后HPLC分析法和香草醛硫酸法简便、快速、精密度、稳定性、重现性、专属性好,适合作为不同类型鞣质的测定方法;地榆乙酸乙酯萃取物的可水解鞣质及缩合鞣质最高。AIM To establish the quantitative assay of hydrolysable tannins and condensed tannins,and in the aqeous extract of Sanguisorba officinalis L and its different solvents partitioned extracts.METHODS The HPLC was used for the determination of hydrolysable tannins content.The Vanillin-H2SO4 method was employed for the determination of condensed tannins content.RESULTS The hydrolysable tannins content in the aqeous extract,ethyl acetate partitioned extract,n-butanol partitioned extract and water residue were（81.83±2.59） mg/g,（206.80±2.43） mg/g,（163.56±5.69） mg/g,（47.62±1.13）mg/g,respectively;The condensed tannins content was（181.72±1.41） mg/g,（594.52±16.76） mg/g,（476.27±13.33） mg/g,（14.41±0.29） mg/g,respectively.CONCLUSION HPLC analysis in acid hydrolysis and Vanillin-H2SO4 method are simple,rapid,accurate,stable,reproducible,selective,and suitable for determination of tannins.Of all these extracts,the tannins content in ethyl acetate extract layer is the highest.

关 键 词：地榆 可水解鞣质 缩合鞣质 高效液相色谱 香草醛硫酸法 

分 类 号：R284.1[医药卫生—中药学]