大豆GmMYB12B2植物表达载体的构建及转化烟草的研究  

Construction of Plant Expression Vector of Soybean GmMYB12B2 Gene and Transformation in Tobacco

作　　者：李晓薇[1] 张艳[1] 赵旭[2] 钱丹丹[1] 闫帆[1] 张庆林[1] 李景文[1] 王庆钰[1] 

机构地区：[1]吉林大学植物科学学院,长春130062 [2]吉林省产品质量监督检验院,长春130022

出　　处：《中国农学通报》2011年第12期91-95,共5页Chinese Agricultural Science Bulletin

基　　金：转基因生物新品种培育重大专项子课题(2008ZX08004-003);国家自然科学基金面上项目(30971808);吉林省科技发展计划重点项目(20080204);长春市科技局国际科技合作项目(08GH10);"211"三期建设项目资助

摘　　要：为研究大豆MYB转录因子-GmMYB12B2在转基因植物类黄酮代谢中的作用,利用吉林大学植物种质资源与利用研究室前期克隆的GmMYB12B2基因,以PPZP质粒为表达载体,构建了由组成型CaMV35S启动子驱动GmMYB12B2基因的植物表达载体PPZP-GmMYB12B2;然后,采用冻融法转入农杆菌EHA105菌株,通过叶盘法对烟草进行遗传转化。经PCR随机扩增鉴定9株转基因烟草,其中有6株是阳性植株,初步证明已获得转大豆GmMYB12B2基因的烟草。In order to study the application of GmMYB12B2 gene in transgenic plants involving flavonoids metabolism,the plant expression vector PPZP-GmMYB12B2 was constructed,in which the GmMYB12B2gene was driven by the constitutive promoter CaMV35S.The constructed expression vector was transformed into EHA105 strain by freeze-thaw method.Leaf segments of tobacco were inoculated with EHA105 containing plasmid PPZP-GmMYB12B2.The transgenic lines were selected by PCR detection,in nine random transgenic tobaccos,six of them were positive plants.The result showed that GmMYB12B2 gene had been integrated into the genome of tobacco.

关 键 词：GmMYB12B2转录因子 植物表达载体 农杆菌转化 烟草 

分 类 号：S565.1[农业科学—作物学]