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作 者:陈托兄[1]
出 处:《中国农学通报》2011年第12期159-162,共4页Chinese Agricultural Science Bulletin
基 金:国家科技支撑"优质抗逆专用草新品种选育"(2006BAD01A19-2)
摘 要:用采自英国苏克塞斯的海滨碱蓬为材料,将铺有滤纸的培养皿中发芽7天的海滨碱蓬幼苗,移栽到育苗盘中溶液培养,用调整过的Hoagland营养液培养海滨碱蓬21天,用150mmol/L NaCl处理海滨碱蓬0h、6h、12h、24h、36h及48h,测定海滨碱蓬在不同处理时间下根、茎、叶的鲜重、干重及Na+含量。分析Na+吸收速率在48h内的变化趋势。结果表明,不同处理之间海滨碱蓬Na+吸收速率差异不显著。因此,海滨碱蓬在48h内的Na+吸收速率基本保持不变,在与Na+吸收途径有关的试验中,处理时间设为48h不仅可以节省人力物力财力,又可以取得事半功倍的良好研究目的。Seed of Suaeda maritima(L.) were collected from a salt marsh at East Sussex,United Kingdom,in 2003.Seeds were germinated on filter paper and kept in petri dishes and wetted sterile water at 23-28℃.After 7 days of germination,seedlings were carefully transplanted to solution culture with modified Hangland nutrient solution containing:6 mmol/L KNO 3,1 mmol/L NH 4 H 2 PO 4,0.5 mmol/L MgSO 4,0.5 mmol/L Ca(NO 3) 2,92 μmol/L H 3 BO 3,0.6 μmol/L CuSO 4,0.7 μmol/L(NH 4) 6 MO 7 O 24,18 μmol/L MnCl 2,1.6 μmol/L ZnSO 4,60 μmol Fe-citrate.After 21 days in solution culture,then plants were treated with 150 mmol/L NaCl for 0 h,6 h,12 h,24 h,36 h and 48 h.Fresh weight,Dry weight and Na + content of root,stem and leaf were determined according to different treatment time.Na + absorption rate of Suaeda maritime was analyzed.The results showed that Na + absorption rate of Suaeda maritima had no significant differences under different time when plants were treated with 150 mmol/L NaCl.So the treatment time in connection with Na + absorption pathway should be supposed to 48 h.It did not only save on manpower,material resources and financial resources but also get two fold results with half the effort.
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