长白猪甘露聚糖结合凝集素-C基因启动子区的克隆与序列分析  

Cloning and Sequence Analysis of Mannan-binding Lectin C promoter from Landrace

作　　者：楼柏丹[1] 周明[1] 满朝来[1] 王洋[1] 刘玉芬[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《中国农学通报》2011年第14期35-39,共5页Chinese Agricultural Science Bulletin

基　　金：哈尔滨师范大学青年学术骨干资助计划项目"猪甘露聚糖结合凝集素基因多态性与TGEV感染相关性的研究"(08KXQ-01)

摘　　要：为了分析猪甘露聚糖结合凝集素C基因的多态性,根据GenBank上已发表的猪甘露聚糖结合凝集素C基因启动子区序列,设计一对特异性引物,通过RT-PCR法成功地从长白猪肝脏中克隆到MBL-C基因启动子区序列。结果表明扩增序列全长2030bp,与已发表的该序列相比具有3个SNPs位点,即-1636位点为T,-1614位点为A,-251位点为C,在-251位点与高水平表达的pMBL-C启动子区位点不同,从A突变为C。研究为下一步分析pMBL-C的启动子区多态性奠定了基础。In order to analyze the polymorphisms of porcine mannose-binding lectin C（pMBL-C） gene, A pair of primers were designed according to the promoter region from pMBL-C published in GenBank, the promoter region of MBL-C from Landrace ’ s liver was acquired successfully by RT-PCR. The result indicated that there were 2030bp in the sequence, three SNPs were found in the sequence, namely, at the position-1636 T,-1614A,-251C, it had one mutation at the position-251, from A to C. The study will provide base for analyzing promoter polymorphism of pMBL-C.

关 键 词：甘露聚糖结合凝集素-C基因 启动子 克隆 序列分析 长白猪 

分 类 号：S852.4[农业科学—基础兽医学]