人参四物汤对成瘾动物胸腺T细胞亚群增殖及IL-2R的作用研究  

The relationship between IL 2R and proliferation of cell in morphine dependent mice and potential effect of Chinese medicine

作　　者：刘屏[1,2,3] 李宾[1,2,3] 王淑民 陈孟莉 陈世铭[1,2,3] 

机构地区：[1]解放军总医院药理研究室 [2]解放军总医院实验仪器中心 [3]北京同仁医院临床药理室

出　　处：《中国免疫学杂志》1999年第8期364-365,368,共3页Chinese Journal of Immunology

基　　金：国家中医药管理局科研基金

摘　　要：目的：观察吗啡对胸腺细胞ＣＤ４，ＣＤ８细胞周期增殖、ＩＬ２的影响及人参四物汤对其调节作用。方法：小剂量递增给药建立吗啡成瘾动物模型后，采用流式细胞仪分析各组小鼠胸腺ＣＤ４、ＣＤ８细胞ＤＮＡ含量、增殖指数以及ＩＬ２Ｒ的情况。结果：吗啡成瘾组小鼠ＣＤ４、ＣＤ８细胞Ｓ，Ｇ２＋Ｍ期和增殖指数显著低于正常组和人参四物汤组动物，而Ｇ０／Ｇ１期高于后二者；吗啡依赖小鼠ＩＬ２Ｒ表达下降，人参四物汤组动物的Ｔ细胞表面ＩＬ２Ｒ表达有明显增加。结论：吗啡使胸腺细胞ＤＮＡ复制受阻导致功能细胞减少，ＩＬ２Ｒ表达下降。Objective:To observe the affect of morphine on proliferation of cyclic cells and IL 2R in mice, and effect of Ginseng SiWu decoction. Method:Kunming mice model for chronic dependence was developed by s. c. morphine, by flow cytometry the proliferation of cyclic cells and the levels of IL 2R were assay, and the activity of Ginseng SiWu decoction was investigated. Result:The G 0/G 1 phase of T subsets(CD 4,CD 8) were significantly higher and S, G 2+M phase lower, and IL 2R expression in morphine dependence mice than Ns and Ginseng SiWu group. Conclusion:Morphine inhibited the DNA copy of cell cycle of thymus lymphocyte subsets and IL 2R expression, Ginseng SiWu decoction was able to regulate it.

关 键 词：吗啡依赖 人参四物汤 细胞增殖 IL-2R 中药 

分 类 号：R289.5[医药卫生—方剂学] R259.955[医药卫生—中药学]