HPLC测定复方止呕颗粒剂中6-姜酚的含量  

Content Determination of 6-gingerol in Antiemetic Compound Particles by HPLC

作　　者：冯果[1] 刘文[1] 张永萍[1] 马雯雯[2] 冯泳[1] 

机构地区：[1]贵阳中医学院,贵阳550002 [2]中国人民解放军第一医院药剂科,兰州730030

出　　处：《中国实验方剂学杂志》2011年第11期68-70,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省中药现代化专项研究课题(黔科合社字[2008]5025号)

摘　　要：目的:建立复方止呕颗粒剂中6-姜酚含量的测定方法。方法:采用高效液相色谱法测定复方止呕颗粒剂中6-姜酚含量。色谱柱Agilenttc-C18柱(4.6 mm×250 mm,5μm),流动相甲醇-水(65∶35),流速0.8 mL·min-1,柱温30℃,进样量10μL,检测波长280 nm。结果:6-姜酚在0.113 5～2.270 0μg与峰面积呈良好的线性关系,回归方程为Y=540 646X-1 188(r=0.999 9,n=6),平均回收率为99.90%,RSD 1.14%。结论:方法简便可行、重复性好,可作为复方止呕颗粒剂中6-姜酚的含量测定方法。Objective：To establish a method for determining 6-gingerol in Antiemetic compound particles.Method：6-gingerol in decoction of Antiemetic compound particles was determined by HPLC.The column was Agilent TC C18（4.6 mm×250 mm,5 μm） with methanol-water（65∶35） as mobile phase.The flow rate was 0.8 mL·min-1,and the column temperature was at 30 ℃.The detection wavelength was set at 280 nm;injection volume was 10 μL.Result：6-gingerol was linear in the range of 0.113 5-2.270 μg and the regression equation was Y=540 646X-1 188（r=0.999 9,n=6）.The average recovery was 99.90%,RSD 1.14%.Conclusion：The method is simple and reproducible for the determination of 6-gingerol in decoction of Antiemetic compound particles by HPLC.

关 键 词：高效液相色谱 复方止呕颗粒剂 6-姜酚 含量测定 

分 类 号：R284.1[医药卫生—中药学]