HPLC测定不同产地野生与栽培地骨皮中总东莨菪内酯  

Content Determination of Total Scopoletin in Wild and Cultivated Cortex Lycii from Different Areas by HPLC

作　　者：刘伟[1] 郭蕾[1] 崔永霞[1] 陈志红[1] 龚海燕[1] 

机构地区：[1]河南中医学院分析测试中心,郑州450008

出　　处：《中国实验方剂学杂志》2011年第11期73-75,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省教育厅自然科学研究计划项目(2009A360010)

摘　　要：目的:建立反相高效液相色谱测定地骨皮中总东莨菪内酯含量的方法。方法:采用HPLC,Kromasil C18色谱柱,流动相乙腈-1%冰醋酸水溶液(15∶85),流速0.8 mL·min-1,柱温30℃,检测波长345 nm。结果:总东莨菪内酯在6～48 mg.L-1呈良好线性关系(r=0.999 9),平均回收率97.19%(RSD 1.77%)。结论:该方法准确、可靠并且专属性强。Objective：To establish an HPLC method for content determination of total scopoletin in wild and cultivated Cortex Lycii from different areas.Method：The separation was performed on a column C18,the mobile phase was acetonitrile-1% acetic acid（15∶85）;the flow rate was 0.8 mL·min-1;the column temperature was at 30 ℃;the detective wavelength was at 345 nm.Result：The linear range of scopoletin was from 6 to 48 mg·L-1.The average recovery was 97.19%（RSD 1.77%）.Conclusion：The method is reliable,accurate and has a good specificity.

关 键 词：高效液相色谱法 地骨皮 东莨菪内酯 含量测定 

分 类 号：R284.1[医药卫生—中药学]