霜制对苦杏仁中苦杏仁苷在大鼠体内代谢及组织分布的影响  

Effect of Cream Processing on Metabolism and Tissue Distribution of Bitter Almond in Rat

作　　者：房敏峰[1] 付志玲[1] 王启林[1] 郑晓晖[1] 

机构地区：[1]西北大学生命科学学院西部资源生物与现代生物技术教育部重点实验室,西安710069

出　　处：《中国实验方剂学杂志》2011年第11期132-137,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省中医管理局基金项目(陕中医发20072507)

摘　　要：目的:探讨霜制对苦杏仁在大鼠体内的代谢及组织分布影响。方法:分别给SD大鼠注射苦杏仁苷,灌胃苦杏仁生品和霜制品水煎液后,采用HPLC测定血浆及各组织中苦杏仁苷及其代谢产物的血药浓度及组织中的含量,进一步利用LC-MSn鉴定代谢产物的结构。结果:苦杏仁苷注射给药后在大鼠体内主要以原型分布,苦杏仁生品和霜制品灌胃后均以次级代谢产物野樱苷的形式分布;且霜制后野樱苷在大鼠各组织中的分布时间延长,分布量更平均,且呈现2次达峰现象。结论:霜制对苦杏仁在大鼠体内的代谢及组织分布有较大影响,该研究结果为探讨苦杏仁炮制机制积累资料。Objective：To study the influence of cream processing on metabolism and tissue distribution of bitter almond in rat.Method：Amygdalin was set as control group given by mainline,bitter almond and its cream product were given to SD rats by oral,HPLC was employed for the determination of amygdalin and its metabolite in blood,heart,liver,spleen,lung and kidney,and LC-MSn was used for further identification of the metabolite structure.Result：Amygdalin was distributed in the tissues as prototype after given by mainline,while in the groups of oral administration,it was distributed as the secondary metabolite prunasin;and compared to bitter almond crude material,the time of prunasin distribution in the tissues was prolonged after cream processing,and showed two peaks phenomenon.Conclusion：Cream processing have a significant effect on the metabolism and tissue distribution of bitter almond,this provides information for the mechanism of bitter almond＇s processing.

关 键 词：苦杏仁 霜制 代谢 组织分布 

分 类 号：R285[医药卫生—中药学]