不同药物对急性心功能不全大鼠模型作用比较  

Comparison of Cardiac Effects Produced by Dobutamine,Radix Astragali and Deslanoside in Rats with Pentobarbital Sodium-Induced Cardiac Insufficiency

作　　者：胡剑江[1] 马淑骅[1] 马琰岩[1] 高峻虹[2] 付卫星[2] 崔海峰[1] 王毅[1] 喻晓春[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]中国中医科学院针灸研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第11期149-152,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873295);财政部资助中国中医科学院基本科研业务费项目(zx-zzxt-2006-006及zz2007001)

摘　　要：目的:建立稳定戊巴比妥钠致大鼠急性心功能不全模型并比较不同种类抗心功能不全药物的作用差异,籍此探讨该动物模型的适用性。方法:将雄性Wistar大鼠分为模型组、多巴酚丁胺组、去乙酰毛花苷组及黄芪注射液组,采用戊巴比妥钠股静脉给药方法建立大鼠急性心功能不全模型,即时用多巴酚丁胺(100μg·kg-1)、去乙酰毛花苷(0.1 mg·kg-1)或黄芪注射液(4 mL·kg-1)静脉给药干预,全过程中使用四道生理仪监测心电图、左心室内压等指标的变化,观察药物治疗效果1～2 h。结果:使用戊巴比妥钠建立的稳定大鼠急性心功能不全模型,左室内压峰值(LVSP)及左室压最大上升速率(dp.dt max-1)等各项指标降至初始值60%～70%并持续90 min以上。给予多巴酚丁胺治疗后2 min左右LVSP及dp.dtmax-1均明显回升至初始值90%以上(P<0.05),给予黄芪注射液治疗后20 min左右LVSP及dp.dtmax-1也明显回升至初始值90%左右(P<0.05),但使用去乙酰毛花苷注射液治疗后未观察到指标明显改善现象。结论:使用戊巴比妥钠可建立较为稳定大鼠急性心功能不全模型,此模型比较适用于多巴酚丁胺类药物及黄芪注射液的药理及药效研究,而不太适用于去乙酰毛花苷类药物的药效及深入机制研究。Objective：To establish a stable pentobarbital sodium-induced model of acute cardiac insufficiency in the rats and compare the effects of different cardiotonics.Method：Male Wistar rats weighing 220～260 g were randomized into three groups,namely,modeling group,dobutamine group and deslanoside group.The acute cardiac insufficiency model was established in the rats by the intravenous administration of pentobarbital sodium.In dobutamine deslanoside and Astragalus mongholicus groups the rats with cardiac insufficiency were treated with intravenous injection of with dobutamine,deslanoside and radix astragali separately.The left ventricular pressure,heart rate and dp·dtmax-1 were measured using physiological recording system.Result：Pentobarbital sodium injection established a stable model of acute cardiac insufficiency.The cardiac function in the rats with the cardiac insufficiency was significantly improved by the injection of dobutamine and radix astragali.However,no significant alteration was observed in the rats treated with deslanoside injection.Conclusion：A stable model of acute cardiac insufficiency could be established in the rats by administration of pentobarbital sodium.The model is applicable to dobutamine or radix astragali related studies,but not suitable to the studies involving deslanoside.

关 键 词：心功能不全 动物模型 多巴酚丁胺 去乙酰毛花苷 黄芪注射液 

分 类 号：R331[医药卫生—人体生理学]