南蛇藤提取物含药血清对小鼠肝癌Hepa1-6细胞的增殖能力和VEGF-c表达水平的影响  

Effects of Serum Containing Clastrus orbiculatus Extracts on Proliferation and VEGF-c Expression in Hepatoma Cells of Mice

作　　者：员林[1,2] 张华[3] 钱亚云 侯莹 朱耀东 马慧 郭试瑜[4] 久光正[4] 刘延庆 

机构地区：[1]扬州大学中西医结合研究所,江苏扬州225009 [2]中国人民解放军97医院影像科,江苏徐州221004 [3]扬州大学医学院护理系,江苏扬州225009 [4]昭和大学医学部第一生理学教室

出　　处：《中国实验方剂学杂志》2011年第11期157-160,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:制备南蛇藤提取物(Celastrus orbiculatus extracts,COE)兔含药血清,初步研究其对小鼠肝癌Hepa1-6细胞株的增殖能力及血管内皮生长因子-c(VEGF-c)表达水平的影响。方法:取新西兰白兔,以20 mg·kg-1.d-1南蛇藤提取物ig,按通法制备含药血清。设立低、中、高剂量组(分别含有10%,20%,30%的南蛇藤提取物含药血清),作用小鼠肝癌Hepa1-6细胞,同时设阴性对照组及5-氟尿嘧啶(5-fluorouracil,5-Fu)阳性对照组,四甲基偶氮唑盐(MTT)法检测含药血清对细胞增殖的影响,酶联免疫吸附测定(ELISA)法检测细胞培养上清VEGF-c的含量。结果:形态学上,3组COE含药血清均使细胞发生典型的凋亡改变;MTT结果显示COE中、高剂量组的含药血清能抑制Hepa1-6细胞增殖,并呈明显的时效关系;ELISA结果表明COE含药血清作用24 h后,中、高剂量组VEGF-c水平降低,与对照组相比有显著性差异。结论:COE含药血清能够有效地抑制小鼠肝癌Hepa1-6细胞的增殖,并降低细胞VEGF-c的表达水平。Objective：Preparing rabbit serum containing Clastrus orbiculatus extracts（COE）,to investigate preliminarily the effects on proliferation and vascular endothelial growth factor-c（VEGF-c） expression in hepatoma cells of mice（Hepa1-6）.Method：The New Zealand white rabbits received COE with dose of 20 mg·kg-1·d-1 by gavage.The medicated rabbit serum was obtained using serum phamacological approach.Hepa1-6 cells were cultured in media with different dosages of COE-containing serum（the low,medium,high dose groups containing 10%,20%,30% medicated serum respectively）.At the same time,the negative control group and 5-fluorouracil（5-Fu） positive control group were set up.The proliferation inhibition effect of medieated serum containing COE on Hepa1-6 cells was detected by microculture tetrazolium（MTT） assay and the expression level of VEGF-c of Hepa1-6 cells was tested by enzyme linked immunosorbent（ELISA）.Result：The typical morphological changes of apoptosis were observed after incubation with COE-containing serum of each dose.The medium and high dose COE-containing serum could significantly inhibit the proliferation of Hepa1-6 cells time-dependently.The VEGF-c expression level of Hepa1-6 cells treating with medium and high dose COE-containing serum for 24 hours were significantly decreased compared with that of the negative control group（P0.05）.Conclusion：COE-containing serum can significantly inhibit proliferation and VEGF-c expression of Hepa1-6 cells in vitro.

关 键 词：南蛇藤提取物 肝癌 含药血清 增殖 血管内皮生长因子-C 

分 类 号：R285.5[医药卫生—中药学]