黄连、丁香外用药理作用研究  

Pharmacological Effects of External Use of Coptidis Rhizoma and Caryophylli Flos

作　　者：翟华强[1] 王双艳[1] 张硕峰[1] 高明超[1,2] 唐婧姝[3] 郭小文[1] 马长华[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京市兴隆中医院,北京100071 [3]北京大学医学部药学院,北京100191

出　　处：《中国实验方剂学杂志》2011年第11期192-195,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技支撑计划课题(2008BAI53B09)

摘　　要：目的:开展黄连、丁香外用抗炎、镇痛以及治疗口腔溃疡的药效学实验,明确其外用功能。方法:抗炎实验选取60只大鼠,随机分成5组,黄连外用于右足跖部皮内注射1%角叉菜胶的大鼠,用软皮尺测量足跖周长;镇痛实验选取60只小鼠,随机分成5组,小鼠尾部外用丁香,用压痛仪测定小鼠尾部压痛阈值;治疗口腔溃疡实验选取96只大鼠,随机分成8组,大鼠口腔溃疡造模后,黄连、丁香口腔给药,计算溃疡愈合率。结果:抗炎实验显示,黄连外用可抑制角叉菜胶所引起的大鼠足跖肿胀,各剂量组与对照组比较,有显著性差异(P<0.05);镇痛实验显示,丁香外用可明显提高小鼠机械性压痛痛阈值,大、中剂量组与对照组比较,有显著性差异(P<0.05);治疗口腔溃疡实验显示,黄连与丁香外用可明显治疗苯酚所致的口腔溃疡,高、中剂量组与对照组比较,有显著性差异(P<0.05);黄连中剂量组与丁香中剂量组比较,二者有显著性差异(P<0.05),丁香中剂量治疗口腔溃疡效果更为显著。结论:黄连外用具有显著的抗炎作用,丁香外用可以镇痛,黄连、丁香均可治疗口腔溃疡。Objective：To study the pharmacological effects of Coptidis Rhizoma and Caryophylli Flos on anti-inflammatory,analgesic and for treatment of oral ulcers with external use.Method：In the anti-inflammatory experiments 60 rats were randomly divided into 5 groups.Their right feet plantar were intradermally injected with 1% carrageenan and given Coptidis Rhizoma extract externally,the paw circumference was measured with soft rule.In analgesia experiments,60 mice were randomly divided into 5 groups.Their tails were given Caryophylli Flos extract externally,tail tenderness threshold was measured with tenderness instrument.In the oral ulcer experiment,96 rats were randomly divided into 8 groups.After the model of oral ulcers,the rats were given Coptidis Rhizoma or Caryophylli Flos extract externally,the ulcer healing rate was then evaluated with computer calculating.Result：① The anti-inflammatory experiments showed that berberine inhibited topical carrageenan induced rat paw edema,there was significant difference between the dose group and control group（P0.05）.② Analgesic experiments showed that clove could significantly elevate the tenderness mechanical pain threshold.there was significant difference between the large,medium dose groups and control group（P0.05）.③ Experiments showed that treatment of oral ulcers induced by phenol with berberine and Caryophylli Flos could gain significant effect,there was significant difference between the large,medium dose groups and control group（P0.05）;also there was significant difference between the medium dose berberine group and the medium dose Caryophylli Flos group（P0.05）,the effect of Caryophylli Flos was better.Conclusion：External use of Coptidis Rhizoma extract has significant anti-inflammatory effects.External use of Caryophylli Flos extract shows effect of analgesia.External use of Coptidis Rhizoma and Caryophylli Flos together can treat oral ulcers effectively.

关 键 词：黄连 丁香 中药外用 

分 类 号：R285.5[医药卫生—中药学]