头痛宁胶囊对偏头痛模型大鼠中脑和下丘脑c-fos,c-jun基因表达的影响  

Effects of Toutongning Capsule on Expression of c-fos and c-jun in Mesencephalon and Thalamencephalon in Rats with Migraine

作　　者：刘斌[1] 符艳松[1] 王彩霞[1] 

机构地区：[1]河北联合大学附属医院神经内一科,河北唐山063000

出　　处：《中国实验方剂学杂志》2011年第11期195-198,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察头痛宁胶囊对偏头痛模型大鼠中脑导水管周围灰质(PAG)和下丘脑c-fos,c-jun表达的影响,探讨头痛宁胶囊治疗偏头痛的作用机制。方法:健康SD大鼠随机分为:生理盐水组、偏头痛模型组、西比灵(1.04 mg·kg-1)治疗组、头痛宁高、中、低剂量(760,380,190 mg·kg-1)治疗组。sc硝酸甘油(NTG)制备偏头痛大鼠模型,给药后4,6 h 2个时间点取材,用免疫组化方法检测中脑导水管周围灰质(PAG)和下丘脑c-fos,c-jun表达水平。结果:与生理盐水组比较,模型组大鼠中脑导水管周围灰质(PAG)和下丘脑c-fos,c-jun阳性细胞数增多(P<0.01,P<0.01),4 h多于6 h。与模型组比较,头痛宁高、中剂量治疗组和西比灵治疗组大鼠中脑导水管周围灰质(PAG)和下丘脑c-fos,c-jun阳性细胞数减少(均P<0.01)。头痛宁低剂量治疗组无明显变化。结论:头痛宁胶囊可以减少由硝酸甘油诱导的致痛物质c-fos,c-jun的表达,从而抑制中脑导水管周围灰质(PAG)和下丘脑痛觉信息的传递,发挥治疗偏头痛的作用。其中,以头痛宁高、中剂量治疗组作用明显。Objective：By observing the effects of Toutongning capsule on the expression of c-fos and c-jun in periaqueductalgrey（PAG） and thalamencephalon in rats with migraine to discuss the therapeutic mechanism of Toutongning capsule.Method：The SD rats were evenly divided into 6 groups：normal group,model group,Flunarizine（1.04 mg·kg-1）intervention group,high-dose,mid-dose and low-dose Toutongning capsule groups（760,380,190 mg·kg-1）.Migraine model in rats was established by NTG subcutaneous injection.After NTG subcutaneous injection the specimen at 4 hours and 6 hours was obtained.The expression of c-fos and c-jun were detected by immunohistochemistry.Result：The expression of c-fos and c-jun in PAG and thalamencephalon were increased in the model group compared with that in the normal group（all P0.01）.At 4 hours after NTG subcutaneous injection,there was much more expression of c-fos and c-jun compared with those at 6 hours after NTG subcutaneous injection.The expressions of c-fos and c-jun in PAG and thalamencephalon were decreased in the high-dose Toutongning capsule intervention group,mid-dose Toutongning capsule intervention group and Flunarizine intervention group compared with those in low-dose Toutongning capsule group and in the model group（all P0.01）.There was no significant difference between model group and low dose intervention group.Conclusion：By reducing the expression of c-fos and c-jun and restrainning the transmission of pain information in PAG and thalamencephalon,Toutongning capsule could treat migraine.And the effect of high-dose Toutongning capsule and mid-dose Toutongning capsule were much better.

关 键 词：偏头痛 C-FOS C-JUN 头痛宁胶囊 大鼠 

分 类 号：R285.5[医药卫生—中药学]