巴豆生物碱对人肝癌SMMC-7721细胞凋亡及Bax,Bcl-2蛋白表达的影响  

Effect of Crotonis Fructus Alkaloid on Apoptosis of Human Hepatoma SMMC-7721 Cells and Bcl-2/Bax Expression

作　　者：陈武[1] 陈鹏英[1] 刘鹏[1] 廖艳林[1] 

机构地区：[1]武汉市中医医院药学部,武汉430014

出　　处：《中国实验方剂学杂志》2011年第11期199-201,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究巴豆生物碱(croton seed alkaloid,CA)对人肝癌SMMC-7721细胞的诱导凋亡作用并探讨其对凋亡相关蛋白Bax和Bcl-2表达的影响。方法:不同浓度CA分别处理SMMC-7721细胞24～48 h后,MTT法检测细胞的生长活性,流式细胞仪检测细胞凋亡率,Western blot检测凋亡相关蛋白Bax,Bcl-2的表达。结果:10～200 mg.L-1 CA均能抑制SMMC-7721细胞增殖(P<0.05),其抑制率与作用时间及浓度相关;CA作用SMMC-7721细胞24 h后,对照组凋亡率为(0.26±0.14)%,10,50,100,200 mg.L-1 CA组细胞凋亡率分别为(5.85±0.47)%,(17.70±1.23)%,(33.25±2.08)%,(49.52±1.85)%,与对照组比较,差异有统计学意义(P<0.05);Western blot结果提示经CA作用24 h后,随着药物浓度的增加,Bax的表达逐渐增加,而Bcl-2的表达逐渐减少。结论:CA能抑制SMMC-7721细胞的生长并促进其凋亡,其发生与Bcl-2蛋白的减少和Bax蛋白表达增多有关。Objective：To study the effect of Crotonis Fructus alkaloid（CA） on apoptosis and the expression of Bcl-2 and Bax in human hepatoma SMMC-7721 cells.Method：After treatment with CA for 24-48 h,MTT assay was used to determine the cell growth inhibitory rate in vitro,flow cytometry was used to detect the apoptosis of SMMC-7721 cells before and after CA treatment.Western blot was used to detect the expression of Bcl-2 and Bax.Result：CA inhibited the growth of SMMC-7721 cells in a dose-and time-dependent manner.After treatment with 50-200 mg·L-1 CA for 24 h,the number of apoptosis cells was increased dose-dependently from（0.26±0.14）% in the control group to（5.85±0.47）%,（17.70±1.23）%,（33.25±2.08）% and（49.52±1.85）% in CA groups,respectively.By Western blot analysis,we found the level of Bax was up-regulated,whereas,the level of Bcl-2 down-regulated.Conclusion：CA can efficiently inhibit the proliferation and induce apoptosis in SMMC-7721 cells.Its mechanism is likely related to the down-regulation of Bcl-2 and up-regulation of Bax.

关 键 词：巴豆生物碱 细胞凋亡 BAX BCL-2 

分 类 号：R285.5[医药卫生—中药学]