五灵肩周丸镇痛抗炎作用和急性毒性研究  

Analgesic and Anti-inflammatory Effects and Acute Toxicity of Wuling Jianzhou Pellet

作　　者：刘霭明[1] 孔焕育[1] 石光[1] 罗超[1] 曹莹[1] 谢宝平[1] 庞建新[1] 丘玉昌[1] 

机构地区：[1]南方医科大学药学院新药评价中心,广州510515

出　　处：《中国实验方剂学杂志》2011年第11期202-204,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究五灵肩周丸的镇痛抗炎作用及急性毒性。方法:采用小鼠扭体实验和甲醛致痛模型来观察五灵肩周丸的镇痛作用;抗炎实验采用大鼠棉球肉芽肿实验及小鼠二甲苯致耳廓肿胀模型;采用小鼠单次ig的方法测定其LD50。结果:给予3.8,7.6 g·kg-1的五灵肩周丸能明显减少醋酸所致小鼠的扭体次数(P<0.05),抑制二甲苯引起的小鼠耳肿胀(P<0.01);五灵肩周丸在3.8,11.4 g·kg-1时能明显减轻甲醛致痛实验中小鼠的Ⅰ相疼痛反应(P<0.01),但对甲醛引起的Ⅱ相疼痛反应没有显著影响;剂量为7.6 g·kg-1时对大鼠棉球肉芽肿的形成有明显的抑制作用(P<0.05);急性毒性试验表明,其LD50为148.4 g·kg-1。结论:五灵肩周丸具有明显的镇痛抗炎作用,且具有较高的安全性。Objective：To study the analgesic and anti-inflammatory effects,as well as acute toxicity of the Wuling Jianzhou pellet.Method：Writhing method and formalin test were used in mice to evaluate the analgesic effect.Xylene induced ear swelling in mice and formation of cotton ball granuloma in rats were adopted to investigate the anti-inflammatory effect.LD50was assessed in mice.Result：At the dose of 3.8,7.6 g·kg-1,Wuling Jianzhou pellet significantly reduced the number of writhing induced by acetic acid（P0.05）,as well as mouse ear swelling induced by xylene（P0.01）.In formalin test,pain of phase I but not phase Ⅱ,was reduced after administration of Wuling Jianzhou pellet at 3.8,11.4 g·kg-1（P0.01）.The formation of cotton ball granuloma was inhibited by Wuling Jianzhou pellet at 7.6 g·kg-1（P0.05）.The LD50of Wuling Jianzhou pellet was 148.4 g·kg-1.Conclusion：Wuling Jianzhou pellet has significant analgesic and anti-inflammatory effects,and exhibits low toxicity.

关 键 词：五灵肩周丸 镇痛 抗炎 急性毒性 

分 类 号：R285.5[医药卫生—中药学]