精元康胶囊对化疗致骨髓抑制小鼠骨髓细胞Bcl-2,Bax mRNA表达水平的影响  

Effect of Jingyuankang Capsule on Expression of Bcl-2,Bax mRNA in Bone Barrow Cells of Model Mice of Chemotherapy-induced Myelosuppression

作　　者：蒋立峰[1] 蒋士卿[2] 

机构地区：[1]南京中医药大学,南京210046 [2]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2011年第11期216-219,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十五"科技攻关计划项目(2004BA719A09-0203);河南省自然科学研究项目(2010A360007)

摘　　要：目的:通过观察精元康胶囊对骨髓细胞Bcl-2,Bax mRNA表达水平的影响,以深入探讨该药的作用机制。方法:实时荧光定量PCR检测骨髓抑制模型小鼠骨髓细胞表达Bcl-2,Bax mRNA的情况。结果:Bcl-2 mRNA的表达水平精元康胶囊组最高,Bax mRNA的表达水平精元康胶囊组最低,与模型组比较有显著差异,与空白组比较无明显差异。结论:精元康胶囊能升高抗凋亡基因Bcl-2的表达,同时降低促凋亡基因Bax的表达,对骨髓细胞凋亡相关通路产生影响,可能是精元康胶囊对化疗致骨髓抑制影响的重要机制。Objective：To observe the effect of Jingyuankang capsule on the expression of Bcl-2,Bax mRNA in bone marrow cells of model mice of chemotherapy-induced myelosuppression and make a further study of the mechanism of the medicine.Method：Measuring the expression of Bcl-2,Bax mRNA in bone marrow cells of model mice of myelosuppression with real-time fluorescence quantitative PCR.Result：The Jingyuankang group had the highest expression of Bcl-2 mRNA and lowest expression of Bax mRNA,compared with the model group,the difference was significant,while compared with the control group,there was no significant differences.Conclusion：These results confirmed preliminarily that Jingyuankang capsule could increase the expression of the anti-apoptotic gene Bcl-2 and decrease the expression of proapoptotic gene Bax,through affecting the apoptosis-related pathway to prevent the blood cells from apoptosis caused by chemotherapy,this may be an important mechanism.of Jingyuankang capsule treating the chemotherapy-induced myelosuppression.

关 键 词：精元康胶囊 骨髓抑制 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]