黄芪多糖对大鼠胰岛素抵抗的治疗作用  

Examination of Treatment with Astragalus Polysaccharides on Insulin Resistance in Rats

作　　者：魏学娟[1] 翁孝刚[1] 张一平[1] 梁辉[1] 陈雪辉[1] 王涛[1] 

机构地区：[1]新乡医学院第一附属医院内分泌科,河南卫辉453100

出　　处：《中国实验方剂学杂志》2011年第11期238-242,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技厅项目(224630170)

摘　　要：目的:观察不同剂量黄芪多糖对高脂饮食诱导的大鼠胰岛素抵抗的治疗作用及机制。方法:将成年雄性SD大鼠随机分为正常对照组(NC组,12只)和高脂模型组(HFM组,60只)。模型建立成功后,大鼠随机分组:高脂对照组(HFC组,12只)、吡格列酮组(Pio组,12只)、黄芪多糖(APS)低、中、高剂量组(即LAPS,MAPS,HAPS组,每组均12只),分别给予生理盐水、20 mg·kg-1.d-1的Pio和200,400,800 mg·kg-1.d-1的APS干预8周。随机选取各分组中的一半大鼠进行高胰岛素-正葡萄糖钳夹实验,以稳态期的葡萄糖输注率(GIR)判定周围组织的胰岛素敏感性。同时测定另一半大鼠空腹血糖(FBG),血浆胰岛素(FINS),游离脂肪酸(FFA),血浆脂连素(APN),肿瘤坏死因子(TNF-α)的水平。结果:与NC组比较,HFC组大鼠血浆FINS,FFA,TNF-α的水平显著升高(P<0.05),血浆APN水平和GIR显著降低(P<0.05);与HFC组比较,LAPS,MAPS组相关指标显著改善(P<0.05)。结论:适当剂量的黄芪多糖可以提高肥胖大鼠的胰岛素敏感性,其机制可能与其降低血浆TNF-α,FFA水平和升高APN水平有关。Objective：To study the mechanisms by which APS ameliorates insulin resistance,we examined whether treatment with APS improves insulin sensitivity in insulin-resistant rats and whether this is associated with an increase of adiponectin and reduction of tumor necrosis alpha（TNF-α） and free fatty acides（FFA） in serum.Method：Seventy male SD rats were randomly divided into two groups：normal control group（NC group,n=12） and high fat-diet-induced model group（HFM group,n=60）.Rats in NC group were fed with ordinary diet while those in HFM group were fed with high fat diet for six weeks.Then the rats in HFM group were randomly divided into five groups：high fat-diet-induced control group（HFC group）,pioglitazone group（Pio group）,low APS group（Laps group）,medium APS group（Maps group） and high APS group（Haps group）,with 12 rats in each.Normal saline,20 mg·kg-1·d-1 pioglitazone and APS of different dosages（200,400,800 mg·kg-1·d-1）were lavaged respectively for eight weeks.Changes of fasting blood glucose（FBG）,fasting insulin（FINS）,adiponectin,TNF-α and FFA in one half rats of each group were routinely measured,meanwhile glucose infusion rate（GIR） of another half was evaluated by hyperinsulinaemic-euglycaemic clamp technique.Result：Compared with those in NC group,the FFA,FINS and TNF-α of the rats in HFC group were significantly higher（P0.05）,while adiponectin and GIR significantly lower（P0.05）.Compared with those in HFC group,the above mentioned indexes in the low and medium APS groups were improved significantly（P0.05）.Conclusion：These results indicate that moderate APS can regulate part of the insulin signaling in insulin-resistant serum and that APS could be a potential insulin sensitizer for the treatment of insulin resistance.

关 键 词：高胰岛素-正葡萄糖钳夹技术 黄芪多糖 脂联素 肿瘤坏死因子-Α 游离脂肪酸 

分 类 号：R285.5[医药卫生—中药学]