棉隆及氯化苦对几种生姜土传病原物的毒力  

Toxicity of dazomet and chloropicrin to soil-borne pathogens of ginger

作　　者：杨晓楠[1] 高德良[1] 韩君[1] 刘峰[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《农药学学报》2011年第3期331-334,共4页Chinese Journal of Pesticide Science

基　　金："十一五"国家科技支撑计划项目(2006BAD08A03)

摘　　要：以侵染生姜的茎腐病菌Pythium myriotylum Drechsler、姜瘟病菌Ralstonia solanacearum和南方根结线虫Meloidagrne incognila为指示病原物,比较了两种土壤处理剂棉隆和氯化苦的熏蒸毒力。采用密闭玻璃容器熏蒸,结合菌丝生长速率法测得棉隆和氯化苦对生姜茎腐病菌的EC50值分别为0.284和0.096 mg/L,EC90值分别为18.106和0.312 mg/L。通过密封盘内摇床振荡(25℃,45 r/min)培养(24 h),结合菌悬液比色法评价了两药剂对姜瘟病菌的毒力。结果表明:氯化苦的活性较高,EC50和EC90值分别为0.176和1.280 mg/L,而棉隆的毒力较低,其EC50和EC90值分别达1 984和5 701 mg/L。采用线虫悬浮液密闭熏蒸法处理48 h,发现棉隆对南方根结线虫的EC50和EC90值分别为0.175和0.634 mg/L,氯化苦毒力略低于棉隆,其EC50和EC90值分别为1.211和3.772 mg/L。The susceptibility of Pythium myriotylum Drechsler isolated from ginger to dazomet and chloropicrin was tested using the mycelium growth rate method in the bell jar.The results showed that the EC50 values of dazomet and chloropicrin were 0.284 and 0.096 mg/L,and the EC90 values were 18.106 and 0.312 mg/L,respectively.After the suspension of Ralstonia solanacearum were inoculated in sealed plates and incubating in a shaker（25 ℃,45 r/min） for 24 h,the toxicity of the two soil fumigant were appraised using the colorimetric method.The results showed that the toxicity of chloropicrin to R.solanacearum was higher than that of dazomet,with EC50 and EC90 values as 0.176 and 1.280 mg/L,however,the EC50 and EC90 values of dazomet were 1 984 and 5 701 mg/L.When fumigating the nematode suspension for 48 h in the chromatography jars,the results of microscopic examination showed that the effects of chloropicrin to Meloidogyne incognita were lower than that of dazomet,the EC50 values were 1.211 and 0.175 mg/L,and the EC90 values were 3.772 and 0.634 mg/L,respectively.

关 键 词：棉隆 氯化苦 土传病原菌 南方根结线虫 毒力 

分 类 号：S482.6[农业科学—农药学] S482.2[农业科学—植物保护]