毛细管电泳-场强放大堆积技术测定白花丹参中的水溶性有效成分  

Capillary electrophoresis with field-enhanced stacking for determination of Water-Soluble Active Principles in Salvia miltiorrhiza var. miltiorrhiza f. alba

作　　者：李玉琴[1] 崔英杰[2] 赵晓明[2] 贾宝秀[1] 齐永秀[1] 

机构地区：[1]泰山医学院药学院,山东泰安271016 [2]泰山医学院动脉粥样硬化研究所,山东泰安271000

出　　处：《中国中药杂志》2011年第11期1466-1470,共5页China Journal of Chinese Materia Medica

基　　金：山东省教育厅项目(J09LF30)

摘　　要：目的:利用高效毛细管电泳-场强放大柱内堆积技术分离测定白花丹参中的水溶性有效成分原儿茶醛,原儿茶酸,丹参素,迷迭香酸和丹酚酸B。方法:采用未涂层熔融石英毛细管柱(75μm×50.2 cm,40 cm),以含15%甲醇的20 mmol.L-1硼砂(pH 10.0)为运行缓冲液,分离电压28 kV,柱温25℃,检测波长210 nm。进样前压力进水3.4 kPa;电动进样-8kV×3 s。结果:原儿茶醛的线性范围是3.0～60.0 mg.L-1(R2=0.999 6);原儿茶酸,丹参素,迷迭香酸,丹酚酸B的线性范围均为1.0～20.0 mg.L-1(R2=0.999 1,0.999 4,0.998 9,0.999 8),检测限分别为0.55,0.40,0.25,0.32,0.38μg.L-1,富集倍数高,灵敏度高;将此方法应用于实际样品测定,回收率为97.31%～99.81%。结论:该方法简单、快速、准确、灵敏度高,可用于白花丹参的质量控制。A simple and sensitive capillary electrophoresis method with field-enhanced stacking concentration for the analysis of protocatechuic aldehyde,protocatechuic acid,danshensu,rosmarinic acid and salvianolic acid B in Salvia miltiorrhiza var.miltiorrhiza f.alba was developed.The separation was achieved with a fused-silica capillary（75 μm×50.2 cm,effective length was 40 cm） and a running buffer 15 mmol·L-1 borax（pH 10.0） containing 20% CH3OH.The UV detection wavelength was 210 nm.The applied voltage was 28 kV,and the cartridge temperature was 25 ℃.Water plug was introduced from the anode by 0.5 psi×4 s before injection.Sample was injected by electrokinetic injection-8 kV×3 s.The linear range of protocatechuic aldehyde is 3.0-60.00 mg·L-1（R2=0.999 8）;that of protocatechuic acid,danshensu,rosmarinic acid and salvianolic acid B are 1.0-20.00 mg·L-1（R2 are 0.999 1,0.999 4,0.998 9 and 0.999 8,respectively）,and the limits of detection of five analyts are 0.55,0.40,0.25,0.32,0.38 μg·L-1,respectively,Stacking factor is higher and precision is satisfactory.The recoveries ranges were from 97.3% to 99.8%.The proposed method was used to determine the protocatechuic aldehyde,protocatechuic acid,danshensu,rosmarinic acid and salvianolic acid B in S.miltiorrhiza var.miltiorrhiza f.alba.The proposed method is simple,rapid,accurate and high sensitivity,and can be used to control of the quality of S.miltiorrhiza var.miltiorrhiza f.alba.

关 键 词：毛细管电泳 场强放大样品堆积 白花丹参 水溶性有效成分 

分 类 号：R284.1[医药卫生—中药学]