HPLC同时测定苦豆草中7种生物碱的含量  

Simultaneous determination of 7 alkaloid in Herba Sophorae Alopecuroidis by HPLC

作　　者：马玲 王俊卿 田杰 王坤 李巍[3] 王英华 

机构地区：[1]宁夏回族自治区药品检验所,宁夏银川75001 [2]宁夏回族自治区中医研究院,宁夏银川75004 [3]日本东邦大学

出　　处：《中国中药杂志》2011年第11期1483-1486,共4页China Journal of Chinese Materia Medica

基　　金：宁夏"十一五"科技攻关项目(宁科计字[2006]74号)

摘　　要：目的:建立了用HPLC对苦豆草中苦豆碱、槐定碱、氧化苦参碱、氧化槐果碱、苦参碱、槐果碱和莱曼碱同时定量的方法。方法:采用HPLC检测方法,色谱柱W aters X-B rige C18(4.6 mm×250 mm,5μm);流动相为乙腈-0.05 mol.L-1的磷酸二氢钾溶液(2.0 mL.L-1三乙胺),进行梯度洗脱;检测波长为205 nm,柱温25℃,进样量10μL,流速1.0 mL.m in-1。结果:苦豆碱、槐定碱、氧化苦参碱、氧化槐果碱、苦参碱、槐果碱和莱曼碱的浓度与峰面积,分别在20.66～103.32μg(r=0.998 8),22.82～114.12μg(r=0.999 7),25.10～125.52μg(r=0.999 1),23.88～119.40μg(r=0.997 5),5.00～24.99μg(r=0.998 6),4.69～23.46μg(r=0.999 6)和4.60～23.01μg(r=0.997 8)范围呈良好的线性关系;平均加样回收率分别为97.12%,97.47%,96.21%,94.64%,98.04%,96.24%和101.31%;RSD分别为7.3%,3.0%,4.5%,5.2%,5.4%,5.8%,4.3%。结论:该方法准确、简便,灵敏,为苦豆草的质量评价提供了依据。Objective：To establish a method for determination of 7 alkaloid in Herba Sophorae Alopecuroidis by HPLC.Method：X-Brige C18（4.6 mm×200 mm,5 μm） column was used with acetonitriles-0.05 mol·L-1 KH2PO4 solution（2.0 mL·L-1 triethylamine） with gradient elution as the mobile phase and 1.0 mL·min-1 as the flow rate.The detection wavelength was 205 nm.Result：Aloperin curve was linear in the range from 20.66 to 103.32 μg（r=0.998 8）and the average recovery was 97.12%（RSD 7.3%）;sophoridine curve was linear in the range from 22.82 to 114.12 μg（r=0.999 7）and the average recovery was 97.47%（RSD 3.0%）;oxymatrine curve was linear in the range from 25.10 to 125.52 μg（r=0.999 1）and the average recovery was 96.21%（RSD 4.5%）;oxysophocarpine curve was linear in the range from 23.88 to 119.40 μg（r=0.997 5）and the average recovery was 94.64%（RSD 5.2%）;matrine curve was linear in the range from 5.00 to 24.99 μg（r=0.998 6）and the average recovery was 98.04%（RSD 5.4%）;sophocarping curve was linear in the range from 4.69 to 23.46 μg（r=0.999 6）and the average recovery was 96.24（RSD 5.8%）;lehmannine curve was linear in the range from 4.60 to 23.01 μg（r=0.997 8）and the average recovery was 101.31%（RSD 4.3%）.Conclusion：The method is accurate,simple and feasible.It can be used as a quality evaluation in Herba S.Alopecuroidis.

关 键 词：苦豆草 苦豆碱 槐定碱 氧化苦参碱 氧化槐果碱 苦参碱 槐果碱 莱曼碱 HPLC 

分 类 号：R284.1[医药卫生—中药学]